Background . Liposomal drug delivery systems are increasingly used in clinical practice due to their ability to improve the pharmacokinetic profile and reduce the systemic toxicity of drugs. Quinacrine is a promising drug with proven cardioprotective and antiviral activity, however, its use is limited by side effects. The development of a liposomal form of quinacrine (QLPS) can overcome these limitations and increase the efficiency of targeted delivery. Objective . To study the physicochemical properties of synthesized liposomal quinacrine with different concentrations of phospholipids, the kinetics of the release of the active substance and biodistribution in vivo. Design and method. Commercially available reagents were used: phospholipids, cholesterol, vitamin E. The physicochemical characteristics of liposomes (hydrodynamic diameter, polydispersity, zeta potential) were stud ied using the coordination light scattering method. The morphology of liposomes was studied using transmission electron microscopy. The biodistribution study was carried out on laboratory mice using in vivo fluorescence imaging. Results . In the course of the work, the physicochemical characteristics of liposomes were studied, two samples with different concentrations of phospholipids were compared, their release profile and biodistribution were described. Conclusion . The developed liposomal quinacrine has optimal physicochemical characteristics. The obtained data on the active substance release profiles and the features of biodistribution in vivo are the basis for the further development of effective and safe drugs on this platform.