Objective. To analyze the advantages and disadvantages of using the average and maximum amplitudes of the frequency ranges in the determination of the spectral parameters of laser Doppler flowmetry. Methods. The study included 20 patients with type 2 diabetes mellitus and 20 patients without diabetes mellitus. Each patient underwent 5-minute registration of LDF. Spectral indices were calculated in two ways: using the average and maximum amplitudes of certain frequency ranges. Results. In both groups, significantly lower values of the contribution of slow-wave flaxmotions, respiratory flaxmotions and flaxmotion index were obtained using maximum amplitudes (p <0.05). At the same time, values of pulse flaxmotions contribution and intravascular resistance in both groups were significantly higher when using maximum amplitudes (p <0.05). The decrease in the contribution of slow-vave flaxmotions and flaxmotion index reflects dysfunction of microcirculation. Thus, to increase the sensitivity of LDF diagnostics, it seems more advantageous to use maximum amplitudes. However, the differentiating symptom of microcirculatory dysfunction according to the diabetic type is the predominance of the contribution of respiratory fluxmotions over the pulse flaxmotions. Therefore, for the differential diagnosis of diabetic microangiopathy, it is reasonable to use methods that allow one to obtain higher values of the contribution of respiratory and lower values of pulse flaxmotion, which is obtained by using medium amplitudes. Conclusion. Calculation methods using the maximum amplitudes of the characteristic frequency ranges of the LDF spectrum have the greatest potential for identifying dysfunction of the microcirculatory blood flow. In contrast, average amplitudesusing methods can improve the differential diagnosis of diabetic microangiopathy. Thus, it seems reasonable to consider the possibility of using both maximum and average amplitudes simultaneously to increase the sensitivity and specificity of LDF diagnostics for using LDF in the diagnosis of microcirculation disorders.