TY - JOUR

T1 - Новые подходы к формированию покрытий на основе альбумина и наночастиц золота для хирального разделения методом капиллярного электрофореза

AU - Моргачева, Виолетта Павловна

AU - Макеева, Дарья Валерьевна

AU - Колобова, Екатерина Алексеевна

AU - Соловьева, Елена Викторовна

AU - Карцова, Людмила Алексеевна

PY - 2023

Y1 - 2023

N2 - , Combination of properties of gold nanoparticles and bovine serum albumin is promising for the formation of chiral stationary phases enabling high enantioselectivity due to developed surface of coatings and increased chiral selector concentration on the capillary walls. In this work we proposed and compared two approaches to the formation of physically adsorbed multilayer coatings based on citrate-stabilized gold nanoparticles (cGNP) and bovine serum albumin (BSA) for the chiral separation in capillary electrophoresis. In the first approach pre-synthesized cGNP modified with BSA were immobilized on the capillary coated by poly(diallyldimethylammonium chloride) (PDADMAC). PDADMAC polymer was used as a binding layer promoting sorption of the nanoparticles on the capillary surface. It was shown that cGNP-BSA was poorly adsorbed on the capillary surface due to low zeta potential and could not be used for the formation of dense coatings. The second approach included sequential layer-by-layer deposition of PDAMAC and cGNP, resulting in the formation of a dense layer of nanoparticles. The main stage included in-capillary functionalization of cGNP with BSA. Scanning electron microscopy confirmed that the layer-by-layer deposition of modifiers ensured the formation of dense layer of nanoparticles on the capillary surface. The coating was stable in the entire range of pH studied (2–10). The application of such coating allowed reduction of BSA concentration (to 5 μM) in the background electrolyte required for the tryptophan enantiomers separation. It confirms the prospectiveness of combining nanoparticles and chiral selectors for the increase of specific surface area of the capillary inner walls.

AB - , Combination of properties of gold nanoparticles and bovine serum albumin is promising for the formation of chiral stationary phases enabling high enantioselectivity due to developed surface of coatings and increased chiral selector concentration on the capillary walls. In this work we proposed and compared two approaches to the formation of physically adsorbed multilayer coatings based on citrate-stabilized gold nanoparticles (cGNP) and bovine serum albumin (BSA) for the chiral separation in capillary electrophoresis. In the first approach pre-synthesized cGNP modified with BSA were immobilized on the capillary coated by poly(diallyldimethylammonium chloride) (PDADMAC). PDADMAC polymer was used as a binding layer promoting sorption of the nanoparticles on the capillary surface. It was shown that cGNP-BSA was poorly adsorbed on the capillary surface due to low zeta potential and could not be used for the formation of dense coatings. The second approach included sequential layer-by-layer deposition of PDAMAC and cGNP, resulting in the formation of a dense layer of nanoparticles. The main stage included in-capillary functionalization of cGNP with BSA. Scanning electron microscopy confirmed that the layer-by-layer deposition of modifiers ensured the formation of dense layer of nanoparticles on the capillary surface. The coating was stable in the entire range of pH studied (2–10). The application of such coating allowed reduction of BSA concentration (to 5 μM) in the background electrolyte required for the tryptophan enantiomers separation. It confirms the prospectiveness of combining nanoparticles and chiral selectors for the increase of specific surface area of the capillary inner walls.

KW - наночастицы золота

KW - БЫЧИЙ СЫВОРОТОЧНЫЙ АЛЬБУМИН

KW - поли(диаллилдиметиламмоний хлорид)

KW - капиллярный электрофорез

KW - полислойные покрытия

KW - bovine serum albumin

KW - capillary electrophoresis

KW - chiral separation

KW - gold nanoparticles

KW - multilayer coatings

KW - poly(diallyldimethylammonium chloride)

UR - https://www.mendeley.com/catalogue/6fb54a45-70b0-3d31-8f38-2144520a377e/

U2 - 10.15826/analitika.2023.27.1.002

DO - 10.15826/analitika.2023.27.1.002

M3 - статья

VL - 27

SP - 21

EP - 31

JO - АНАЛИТИКА И КОНТРОЛЬ

JF - АНАЛИТИКА И КОНТРОЛЬ

SN - 2073-1442

IS - 1

ER -