Диагностическая значимость определения концентрации свободных легких цепей иммуноглобулинов в слюне при первичном синдроме Шегрена. / Kuvardin, E. S.; Kholopova, I. V.; Belyakova, E. A.; Grigoryeva, I. N.; Masing, A. V.; Tkachenko, O. Yu.; Bekhtereva, I. A.; Maslyansky, A. L.; Krivolapov, Yu. A.; Lapin, S. V.
In: СОВРЕМЕННАЯ РЕВМАТОЛОГИЯ, Vol. 17, No. 1, 01.02.2023, p. 24–30.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Диагностическая значимость определения концентрации свободных легких цепей иммуноглобулинов в слюне при первичном синдроме Шегрена
AU - Kuvardin, E. S.
AU - Kholopova, I. V.
AU - Belyakova, E. A.
AU - Grigoryeva, I. N.
AU - Masing, A. V.
AU - Tkachenko, O. Yu.
AU - Bekhtereva, I. A.
AU - Maslyansky, A. L.
AU - Krivolapov, Yu. A.
AU - Lapin, S. V.
PY - 2023/2/1
Y1 - 2023/2/1
N2 - Proliferation and hyperactivation of B-lymphocytes in the salivary glands is a feature of primary Sjцgren's syndrome (pSS). Detection in saliva of proteins synthesized by B-lymphocytes may be important in the diagnosis of this disease. Objective: to evaluate the diagnostic value of measuring the concentration of immunoglobulin free light chains (FLC) in saliva in patients with pSS. Material and methods. The cross-sectional study included 24 patients with pSS over the age of 18 years. PSS was diagnosed according to the 2016 ACR/EULAR classification criteria. The control group consisted of 11 healthy volunteers. Blood-salivary glands histohematic barrier permeability ratio for albumin, FLC was measured. Quantitative determination of FLC and in blood and saliva was performed by enzyme immunoassay. An immunohistochemical study of biopsies of minor salivary glands (MSG) was carried out with a quantitative assessment of CD3+, CD4+, CD8+, CD20+, CD21+, CD68+, CD138+ cells. The Mann–Whitney U-test was used to compare quantitative traits. Identification of diagnostic thresholds for the concentration of FLC in saliva for the diagnosis of pSS was carried out using the ROC analysis method. An operating characteristic curve was plotted, the area under the curve, indicators of diagnostic specificity, diagnostic sensitivity, and diagnostic accuracy were calculated. Results and discussion. The obtained values corresponded to the low permeability of the histohematic barrier of the salivary glands for albumin and FLC in patients with pSS and healthy individuals. The median concentrations of FLC κ and λ in the saliva of patients with pSS and healthy volunteers were 1.08 [0.58; 1.91], 1.038 [0.55; 2.03] mg/l and 0.36 [0.32; 0.54], 0.35 [0.21; 0.52] mg/l, respectively. The concentration of FLC in the saliva of patients with pSS was statistically significantly higher than in the control group (p
AB - Proliferation and hyperactivation of B-lymphocytes in the salivary glands is a feature of primary Sjцgren's syndrome (pSS). Detection in saliva of proteins synthesized by B-lymphocytes may be important in the diagnosis of this disease. Objective: to evaluate the diagnostic value of measuring the concentration of immunoglobulin free light chains (FLC) in saliva in patients with pSS. Material and methods. The cross-sectional study included 24 patients with pSS over the age of 18 years. PSS was diagnosed according to the 2016 ACR/EULAR classification criteria. The control group consisted of 11 healthy volunteers. Blood-salivary glands histohematic barrier permeability ratio for albumin, FLC was measured. Quantitative determination of FLC and in blood and saliva was performed by enzyme immunoassay. An immunohistochemical study of biopsies of minor salivary glands (MSG) was carried out with a quantitative assessment of CD3+, CD4+, CD8+, CD20+, CD21+, CD68+, CD138+ cells. The Mann–Whitney U-test was used to compare quantitative traits. Identification of diagnostic thresholds for the concentration of FLC in saliva for the diagnosis of pSS was carried out using the ROC analysis method. An operating characteristic curve was plotted, the area under the curve, indicators of diagnostic specificity, diagnostic sensitivity, and diagnostic accuracy were calculated. Results and discussion. The obtained values corresponded to the low permeability of the histohematic barrier of the salivary glands for albumin and FLC in patients with pSS and healthy individuals. The median concentrations of FLC κ and λ in the saliva of patients with pSS and healthy volunteers were 1.08 [0.58; 1.91], 1.038 [0.55; 2.03] mg/l and 0.36 [0.32; 0.54], 0.35 [0.21; 0.52] mg/l, respectively. The concentration of FLC in the saliva of patients with pSS was statistically significantly higher than in the control group (p
KW - immunoglobulin free light chains
KW - plasma cells
KW - primary Sjцgren's syndrome
KW - saliva
UR - https://www.mendeley.com/catalogue/be719c10-431d-32ab-8669-3fda3782d1d4/
U2 - 10.14412/1996-7012-2023-1-24-30
DO - 10.14412/1996-7012-2023-1-24-30
M3 - статья
VL - 17
SP - 24
EP - 30
JO - Sovremennaya Revmatologiya
JF - Sovremennaya Revmatologiya
SN - 1996-7012
IS - 1
ER -
ID: 114639985