The article puts the first hand-written original of Northern Urals Ethno-Geographic Map by A. Reguli into scientific circulation. The circumstances of its admission to the Cartographic Fund of Russia in connection with preparation of the Ural Expedition by the Russian Geographical Society are considered. This preparation was accompanied by active gathering information on the Ural region. It was obtained from archival documents. There are some interesting facts about arranging the expedition and the interaction of the Society members, such as representatives of the RGS Ethnographic Commission with A. Reguli. The author describes the goals of Reguli’s ethnographic and philological researching in Northern Urals and the conditions of mapping this region. А detailed description of the Ethno-Geographic Map’s contents is given. The analysis of the sources used at the map compilation was performed, and the value of its further use was estimated. Information on existing projects dedicated to the scientific heritage of A. Reguli is provided.