The article data on the study of reflection spectra of hard tooth tissues and dental restoration materials are presented. The method of investigation of dental hard tissues and dental restoration materials - the method of diffuse reflection electron spectroscopy is defined. The relevance of the work, since a high level of restoration aesthetics in therapeutic dentistry is important is shown. Reflection spectra of the studied samples on an automated spectrofluorimeter SDL-2 were performed. Spectral dependences of the reflection coefficient of enamel and dentin sections, as well as intact teeth of patients of different ages, were obtained. The spectral dependences of the reflection coefficient of various dental restoration materials were analyzed.