TY - JOUR

T1 - Резистентные к действию генотоксических факторов многоядерные клетки в культивируемых линиях глиобластом человека

AU - Kiseleva, L. N.

AU - Kartashev, A. V.

AU - Vartanyan, N. L.

AU - Pinevich, A. A.

AU - Samoilovich, M. P.

N1 - Publisher Copyright: © 2018 Sankt Peterburg. All rights reserved. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2018

Y1 - 2018

N2 - Glioblastomas are characterized by significant differences of neoplasms occurring in individual patients, as well as high heterogeneity of cell content within each tumor. The influence of photonic irradiation applied in different modes and doses on the cells of two new glioblastoma cell lines (T2 and R1) and long-studied lines of glioblastoma (T98G and A172) was investigated. In contrast to A172 and R1 cultures, in which all the cells died after exposure to relatively low radiation doses, in T98G and T2 lines a radioresistant cell population survived after higher doses of irradiation. This population was represented by large single or multinuclear non-proliferating growth-arrested cells. These cells remained viable for more than a month and in comparison with the intact cells were characterized by a multiple increase in the expression of genes encoding growth and proangio-genic factors, some extracellular matrix proteins as well as proteins connected with mesenchymal phenotype. The pattern of gene activity of growth-arrested irradiated T98G and T2 cells turned to be identical to gene pattern of the same cells treated with another genotoxic factor — fotemustine. Consequently, growth-arrested resistant cells in T98G and T2 glioblastomas actively expressed genes encoding the proteins that could affect both the tumor cells and their microenvironment.

AB - Glioblastomas are characterized by significant differences of neoplasms occurring in individual patients, as well as high heterogeneity of cell content within each tumor. The influence of photonic irradiation applied in different modes and doses on the cells of two new glioblastoma cell lines (T2 and R1) and long-studied lines of glioblastoma (T98G and A172) was investigated. In contrast to A172 and R1 cultures, in which all the cells died after exposure to relatively low radiation doses, in T98G and T2 lines a radioresistant cell population survived after higher doses of irradiation. This population was represented by large single or multinuclear non-proliferating growth-arrested cells. These cells remained viable for more than a month and in comparison with the intact cells were characterized by a multiple increase in the expression of genes encoding growth and proangio-genic factors, some extracellular matrix proteins as well as proteins connected with mesenchymal phenotype. The pattern of gene activity of growth-arrested irradiated T98G and T2 cells turned to be identical to gene pattern of the same cells treated with another genotoxic factor — fotemustine. Consequently, growth-arrested resistant cells in T98G and T2 glioblastomas actively expressed genes encoding the proteins that could affect both the tumor cells and their microenvironment.

KW - A172

KW - Extracellular matrix protein genes

KW - Fotemustine

KW - Genotoxic factors

KW - Glioblastoma

KW - Growth factor genes

KW - Growth-arrested cells

KW - Irradiation

KW - Mesenchymal markers

KW - Multinuclear cells

KW - R1

KW - Resistant cells

KW - T2

KW - T98G

UR - http://www.scopus.com/inward/record.url?scp=85064645667&partnerID=8YFLogxK

U2 - 10.31116/tsitol.2018.08.05

DO - 10.31116/tsitol.2018.08.05

M3 - статья

AN - SCOPUS:85064645667

VL - 60

SP - 616

EP - 622

JO - Цитология

JF - Цитология

SN - 0041-3771

IS - 8

ER -