Research output: Contribution to journal › Article › peer-review
Split injection of samples into capillary gas chromatographic columns is almost always accompanied by distortions (discrimination) of their composition, especially at a low split ratio. The principal effect is the dependence of absolute peak areas of various constituents vs. the injector temperature. It makes impossible comparing the analytical results for the samples in different solvents and at various injection temperatures. Suppressing these effects is possible only by using splitless injection technique or packed chromatographic columns. However, since the absolute peak areas distortions of various constituents of the same samples are similar, the ratios of peak areas of target components to the peak areas of the solvent appear to be close to constants. Hence, using these relative peak areas instead of absolute values allows compensating the manifestations of discrimination effects during the split injections into capillary columns, but that does not mean the elimination of these effects. The possibilities of the considered approach were characterized on the example of the solution of model analytes in polar high boiling solvent - 1,2-propanediol - within the injector temperature range from 120 to 240°C. It is concluded that average quantification errors in this case by the method of standard addition are about -1.3% rel. Moreover, it allowed changing the injector temperature without decreasing the precision of the results that was previously considered impossible.
Translated title of the contribution | Compensation of discrimination effects of split injection into capillary columns |
---|---|
Original language | Russian |
Pages (from-to) | 110-119 |
Number of pages | 10 |
Journal | АНАЛИТИКА И КОНТРОЛЬ |
Volume | 23 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2019 |
ID: 48586169