The role of serotonin in the recovery of motor functions in spinal cord injuries is intensively studied, but the mechanism of its action remains unclear. In this work, we used the preparation of an isolated segment of the spinal cord of an adult frog to compare the electrophysiological properties of damaged and intact lumbar motoneurons and the modulating effect of serotonin (5-HT) on them. Due to specific morphology of the motoneurons (a very branched dendritic tree), we could reliably obtain damaged (on the surface of the slice) and intact motoneurons (in the depth of the slice). Using intracellular recording, we found significant differences between these groups of neurons in the resting membrane potential, input resistance, properties of the action potential (amplitude, duration, fast and medium phases of the afterhyperpolarization), the frequency of spikes. We found that 5-HT reduced the amplitude of the afterhyperpolarization and increased the frequency of spikes in intact neurons, whereas in damaged mo