In this study, we analyzed distribution of nicotinic acetylcholine receptors, the degree of fragmentation of endplates and their area, and membrane localization of the Na,K-ATPase a2 isoform in the rat soleus muscle after functional unloading (the method of hindlimb suspension) for 1-3 days. After unloading, increased endplate fragmentation, accompanied by a decrease in the area of individual fragments was observed; the total area of the endplates was not changed. The distribution density of nicotinic acetylcholine receptors also remained unaltered, however, a significant decrease in the distribution density of the Na,K-ATPase a2 isoform was observed. Presumably, identified changes resulted from adaptive skeletal muscle remodeling, developing not only under chronic disorders, but even in the early stages of motor dysfunction.