Healthcare-associated infections (HAI) are one of the most pressing problems facing modern medicine. One of the elements of organization of control of HAI in hospital is microbiological monitoring and assessment of antimicrobial resistance of strains isolated from patients. Of particular concern is the high prevalence of Klebsiella pneumoniae strains. These strains frequently cause urinary tract infections, pneumonia and have multiple resistance to antibacterial drugs. In this regard, the aim of the study is to evaluate the organization of the microbiological monitoring system to improve the internal system of epidemiological safety and control of UTIs in hospitals. In this article, a retrospective analysis of the results of microbiological monitoring of two hospitals in the city of St. Petersburg, carried out in the period from May to October 2024, is carried out. The results of the study confirm the high epidemiologic significance of the group of ESCAPE-pathogens, in particular Klebsiella pneumoniae, and demonstrate their pronounced resistance to antibiotics compared to other relevant strains. The necessity of regular microbiological monitoring for timely correction of empirical therapy protocols, early prescription of rational antibiotic therapy and prevention of the spread of antibiotic-resistant strains in medical institutions was substantiated.