TY - JOUR

T1 - АДЕНОЗИН-РЕГУЛИРУЕМЫЕ МЕХАНИЗМЫ В ПАТОГЕНЕЗЕ ВЕНТИЛЯЦИОННЫХ НАРУШЕНИЙ У БОЛЬНЫХ ТУБЕРКУЛЕЗОМ ЛЕГКИХ

AU - Dyakova, Marina E.

AU - Serebryanaya, N. B.

AU - Kiryukhina, L. D.

AU - Esmedlyaeva, D. S.

AU - Yablonskiy, P. K.

N1 - Publisher Copyright: © 2021 Saint Petersburg Pasteur Institute. All rights reserved.

PY - 2021/9/20

Y1 - 2021/9/20

N2 - Uncovering involvement of the purinergic system in the pathogenesis of ventilation disorders (VD) may provide additional information about the pathophysiological mechanisms leading to the development of VD in pulmonary tuberculosis (PT). The aim was to identify a relationship between the parameters of adenosine metabolism, inflammatory response and altered ventilation metabolism in PT patients. Materials and methods. Obstructive and mixed PT patients were assigned to subgroups with/without VD for assessing adenosine deaminase activity (ADA-1, 2) in serum, mononuclear cells, neutrophils; ecto-5'-nucleotidase (ecto-5'-NT); CD26 (dipeptidyl peptidase-4, DPP-4), phagocyte oxidative burst measured by NO generation. Results. PT patients showed decreased ADA-1 and CD26 (DPP-4), but increased ADA-2. Elevated intracellular adenosine concentration was found in mononuclear cells in patients lacking VD, whereas patients with mixed and obstructive VD - had it in neutrophils. Mononuclear cells of patients with PT lacking VD as well as with obstructive VD type had decreased NO3- concentration. Neutrophil hyperactivity was recorded in all groups of PT patients. Patients with PT lacking VD as well as with mixed VD type showed that the parameters of external respiration were associated with activity of extra-/intracellular ADA, whereas obstructive VD was caused by excessive formation of serum adenosine. Changes in respiratory function in PT were associated with decreased level of serum NO radicals, impaired nitrogen-dependent bactericidal phagocyte activity, and overproduced neutrophil oxygen radicals. Conclusion. Purinergic regulation is involved in regulating inflammatory and compensatory processes in PT patients as well as impaired ventilation efficiency. The most severe respiratory disorders observed in PT patients with mixed VD type are associated with the most prominent changes in nucleotidase activity, particularly ecto-ADA-2 and DPP-4/CD26.

AB - Uncovering involvement of the purinergic system in the pathogenesis of ventilation disorders (VD) may provide additional information about the pathophysiological mechanisms leading to the development of VD in pulmonary tuberculosis (PT). The aim was to identify a relationship between the parameters of adenosine metabolism, inflammatory response and altered ventilation metabolism in PT patients. Materials and methods. Obstructive and mixed PT patients were assigned to subgroups with/without VD for assessing adenosine deaminase activity (ADA-1, 2) in serum, mononuclear cells, neutrophils; ecto-5'-nucleotidase (ecto-5'-NT); CD26 (dipeptidyl peptidase-4, DPP-4), phagocyte oxidative burst measured by NO generation. Results. PT patients showed decreased ADA-1 and CD26 (DPP-4), but increased ADA-2. Elevated intracellular adenosine concentration was found in mononuclear cells in patients lacking VD, whereas patients with mixed and obstructive VD - had it in neutrophils. Mononuclear cells of patients with PT lacking VD as well as with obstructive VD type had decreased NO3- concentration. Neutrophil hyperactivity was recorded in all groups of PT patients. Patients with PT lacking VD as well as with mixed VD type showed that the parameters of external respiration were associated with activity of extra-/intracellular ADA, whereas obstructive VD was caused by excessive formation of serum adenosine. Changes in respiratory function in PT were associated with decreased level of serum NO radicals, impaired nitrogen-dependent bactericidal phagocyte activity, and overproduced neutrophil oxygen radicals. Conclusion. Purinergic regulation is involved in regulating inflammatory and compensatory processes in PT patients as well as impaired ventilation efficiency. The most severe respiratory disorders observed in PT patients with mixed VD type are associated with the most prominent changes in nucleotidase activity, particularly ecto-ADA-2 and DPP-4/CD26.

KW - Adenosine

KW - Adenosinedeaminase

KW - Nitric oxide radicals

KW - Oxygen

KW - Purinergic system

KW - Tuberculosis

KW - Ventilation disorders

KW - CELLS

KW - DEAMINASE

KW - EXTRACELLULAR ATP

KW - INFLAMMATION

KW - SOLUBLE CD26

KW - STANDARDIZATION

KW - adenosine

KW - adenosinedeaminase

KW - oxygen and nitric oxide radicals

KW - purinergic system

KW - tuberculosis

KW - ventilation disorders

KW - Adenosine

KW - Oxygen

KW - Ventilation disorders

KW - Nitric oxide radicals

KW - Purinergic system

KW - Tuberculosis

KW - Adenosinedeaminase

UR - http://www.scopus.com/inward/record.url?scp=85116698138&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d48b9b46-439b-3b34-9453-2417984d1788/

U2 - 10.15789/2220-7619-ARM-1475

DO - 10.15789/2220-7619-ARM-1475

M3 - статья

AN - SCOPUS:85116698138

VL - 11

SP - 671

EP - 682

JO - Russian Journal of Infection and Immunity

JF - Russian Journal of Infection and Immunity

SN - 2220-7619

IS - 4

ER -