<p num="22">FIELD: medicine.</p> <p num="23">SUBSTANCE: invention refers to medicine, namely to pediatric surgery, and can be used for children with Hirschsprung's disease in perioperative period with antibacterial prevention. Preoperative preparation involves determination of carriers of toxigenic strains C.difficile, for this purpose, admission to the hospital is followed by examination of faeces for toxigenic strains C.difficile. If C.difficile toxins A and B (or A+B) are detected, an antibacterial therapy is prescribed: vancomycin 10 mg/kg for admission 4 times day for 7 days. Simultaneously, a probiotic preparation is administered based on Saccharomyces boulardii 250 mg once day for 7 days. In intraoperative period 60 minutes before the operation, metronidazole 7.5 mg/kg is intravenously administered. 30–40 minutes before the operation intravenous introduction of cefazolin 25 mg/kg. If the surgical intervention is continued for more than 3–4 hours, one more dose of cefazolin is added. Either 120 minute