The study focuses on revealing the patterns of postmigrational development of neurons accompanying the stratification of the dorsal precentral and superior temporal regions of the neocortex of the human fetal brain. We studied 10 fetal brains at 20–26 weeks of gestation. Tissue blocks were embedded in paraffin, serial sections were made, every 10th section was stained according to Nissl. The rest were immunostained with antibodies to MAP2, layer-specific proteins SATB2, FOXP1 and CTIP2, Reelin and N200. In 4 cases, additional blocks were not embedded in paraffin, but treated according to either Clarity optical tissue clearing protocol or MALDI IMS methods. From 20 to 26 weeks of prenatal development the cortical plate of the studied areas of the cortex contained neurons immunopositive to antibodies of layer-specific transcription factors which were confined to different levels of the cortical plate: SATB2+ – to the upper, FOXP1+ and CTIP2+ – to the lower level. A quantitative assessment of maximum density of SATB2+, FOXP1+, and CTIP2+ neurons made it possible to separate embryonic layers eII, eIII, eV, eVI and the subplate. In the studied period, the number of neurons immunopositive to MAP2 in the cortical plate of both areas is steadily rises. The first groups of MAP2+ pyramidal cells was observed in 20-week-old fetuses in layer eV; by 26 weeks, groups of layer eIII MAP2+ pyramidal cells were noted. The results of immunostaining of large blocks of the human fetal cortical cortex indicates a complex spatial organization of the marginal zone, with the localization of Reelin-positive Cajal-Retzius cells above the level of the plexus fibers. The data obtained in this research indicates that two anatomically remote areas of the cortex – the precentral and superior temporal cortex regions develop synchronously in the first half of the fetal period.