Research output: Contribution to journal › Article › peer-review
ПОЛУЧЕНИЕ И ИССЛЕДОВАНИЕ АКТИВНОСТИ МОДИФИЦИРОВАННЫХ БЕЛКОВ МЕМБРАНЫ МАГНЕТОСОМ. / Gruzdev, D S; Dzuba, M V; Gerasimov, A S.
In: Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 49, No. 3, 26.07.2013, p. 242-8.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - ПОЛУЧЕНИЕ И ИССЛЕДОВАНИЕ АКТИВНОСТИ МОДИФИЦИРОВАННЫХ БЕЛКОВ МЕМБРАНЫ МАГНЕТОСОМ
AU - Gruzdev, D S
AU - Dzuba, M V
AU - Gerasimov, A S
PY - 2013/7/26
Y1 - 2013/7/26
N2 - Genetic constructs coding for the fusion of the Mam12 membrane protein from the magnetotactic bacteria Magnetospirilium magnetotacticum with one B-domain (mb) or two B or Z domains (mbb and mzz, respectively) of Staphylococcus aureus protein A have been synthesized. Expression, fractionation, and purification of fusion proteins have been performed, and the activity of the modified proteins has been characterized. ELISA results showed that all modified proteins displayed immunoglobulin-binding activity.
AB - Genetic constructs coding for the fusion of the Mam12 membrane protein from the magnetotactic bacteria Magnetospirilium magnetotacticum with one B-domain (mb) or two B or Z domains (mbb and mzz, respectively) of Staphylococcus aureus protein A have been synthesized. Expression, fractionation, and purification of fusion proteins have been performed, and the activity of the modified proteins has been characterized. ELISA results showed that all modified proteins displayed immunoglobulin-binding activity.
KW - Gene Expression Regulation, Bacterial
KW - Magnetosomes
KW - Magnetospirillum
KW - Membrane Proteins
KW - Staphylococcal Protein A
KW - Staphylococcus aureus
KW - English Abstract
KW - Journal Article
M3 - статья
C2 - 23882942
VL - 49
SP - 242
EP - 248
JO - ПРИКЛАДНАЯ БИОХИМИЯ И МИКРОБИОЛОГИЯ
JF - ПРИКЛАДНАЯ БИОХИМИЯ И МИКРОБИОЛОГИЯ
SN - 0555-1099
IS - 3
ER -
ID: 11843702