Abscisic acid (ABA) plays a significant role in many physiological processes and response of plants to abiotic and biotic stresses. Phytopathogenic fungi also produce ABA, but the role of this trait in interactions with host plants is poorly understood. In this work, 65 collection strains of phytopathogenic fungi (13 genera, 25 species) were screened for ABA production in batch culture using modified potato dextrose (MPD) and original chemically defined (OCD) media. Thirty-four strains, which belong to 13 species, produced ABA growing on MPD medium, and nineteen strains among them also produced ABA growing on OCD medium. A maximum ABA concentration was detected in MPD culture fluid of strain Apiospora montagnei MF-R13.8 (56.5 ± 0.1 pg L^-1), whereas strain MF-S41.5 of the same species was the most active ABA producer (13.4 ± 1.1 pg L^-1) growing on OCD medium. For the first time, ABA was detected in species Alternaria tenuissima, Apiospora montagnei, Bipolaris sorokiniana, Fusarium avenace