Purpose: to assess effect of eye drops instillation on the refractive power of the cornea and potential intraocular lens (IOL) power calculation errors appearing related within.Patients and methods. There were 478 patients divided in 3 groups: 1st - patients without glaucoma and topical treatment (n = 325); 2nd - individuals with primary open angle glaucoma (POAG) on treatment (n = 122); 3rd - patients with POAG and previous trabeculectomy (TE) without using eye drops (n = 31). For each patient keratometry was performed by IOL-Master 500. Mean preoperative keratometry and corneal astigmatism were compared within IOL calculation error received by Barrett Universal II formula 1 month after phacoemulsification (PE).Results. Mean keratometry was significantly (p < 0.001) less (0.6 D) at the 1st group (44.00 ± 1.96 D) versus 2nd group (44.6 ± 1.76 D). Cancellation of topical eye drops due to TE leads to insignificant decline of corneal curvature (from 44.6 ± 1.76 D to 44.1 ± 1.59 D). There was no any difference in