The study was conducted in 2016 on the four intramire lakes (Khotavets, Zmeinoe, Dubrovskoe, Motykino) in the Darwinskiy State Reserve (Vologda Region). The estimated macrophyte flora of the lakes is 36 species (Rhodophyta - 1, Bryophyta - 5, Equisetophyta - 1, Polypodiophyta -1, and Magnoliophyta - 28, with the 16 dicotyledons and 12 monocotyledons. The flora of individual lakes included from 9 to 23 species. The fol- lowing features were typical of the studied lakes: (1) low total species richness; (2) dominance of typical mire macrophytes; (3) small amount of species common for all study lakes; (4) pvevalence of communities with a low species number (only a few species played a role of a coenosis-forming ones, especially Nuphar lutea (L.) Smith); (5) low degree of overgrowing (as a rule, from 1-3 to 5-7% of water area); (6) overgrowing is influ- enced by the adjoining mires and their sizes, as well as the hydrochemical regimes and morphometric characte- ristics of the lakes. The following species are indicated for the Darwinskiy State Reserve for the first time: Ba- trachospermum turfosum Bory (Lake Dubrovskoe) and Hammarbya paludosa (L.) O. Kuntze (floating mire, Lake Khotavets).