Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation: Synaptic vesicle clustering in a liquid phase

Arndt Pechstein, Nikolay Tomilin, Kristin Walther, Olga Vorontsova, Елена Сергеевна Сопова, Emma Evergren, Volker Haucke, Lennart Brodin, Олег Викторович Шупляков

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Liquid-liquid phase separation is an increasingly recognized mechanism for compartmentalization in cells. Recent in vitro studies suggest that this organizational principle may apply to synaptic vesicle clusters. Here we test this possibility by performing microinjections at the living lamprey giant reticulospinal synapse. Axons were maintained at rest to examine if reagents introduced into the cytosol would enter a putative liquid phase to disrupt critical protein-protein interactions. We found that compounds that perturb the intrinsically disordered region of synapsin, which is critical for liquid phase organization in vitro, cause dispersion of synaptic vesicles from resting clusters. Reagents that perturb SH3 domain interactions with synapsin were found ineffective at rest. Our results indicate that synaptic vesicles at a living central synapse are organized as a distinct liquid phase maintained by interactions via the intrinsically disordered region of synapsin.
Язык оригиналарусский
ЖурналCell Reports
СостояниеПринято в печать - 2019

Ключевые слова

  • synapse
  • synaptic vesicle
  • synapsin
  • accessory proteins
  • phase separation
  • SH3-domain interaction

Цитировать

Pechstein, A., Tomilin, N., Walther, K., Vorontsova, O., Сопова, Е. С., Evergren, E., ... Шупляков, О. В. (Принято в печать). Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation: Synaptic vesicle clustering in a liquid phase. Cell Reports.
Pechstein, Arndt ; Tomilin, Nikolay ; Walther, Kristin ; Vorontsova, Olga ; Сопова, Елена Сергеевна ; Evergren, Emma ; Haucke, Volker ; Brodin, Lennart ; Шупляков, Олег Викторович. / Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation : Synaptic vesicle clustering in a liquid phase. В: Cell Reports. 2019.
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title = "Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation: Synaptic vesicle clustering in a liquid phase",
abstract = "Liquid-liquid phase separation is an increasingly recognized mechanism for compartmentalization in cells. Recent in vitro studies suggest that this organizational principle may apply to synaptic vesicle clusters. Here we test this possibility by performing microinjections at the living lamprey giant reticulospinal synapse. Axons were maintained at rest to examine if reagents introduced into the cytosol would enter a putative liquid phase to disrupt critical protein-protein interactions. We found that compounds that perturb the intrinsically disordered region of synapsin, which is critical for liquid phase organization in vitro, cause dispersion of synaptic vesicles from resting clusters. Reagents that perturb SH3 domain interactions with synapsin were found ineffective at rest. Our results indicate that synaptic vesicles at a living central synapse are organized as a distinct liquid phase maintained by interactions via the intrinsically disordered region of synapsin.",
keywords = "synapse, synaptic vesicle, synapsin, accessory proteins, phase separation, SH3-domain interaction",
author = "Arndt Pechstein and Nikolay Tomilin and Kristin Walther and Olga Vorontsova and Сопова, {Елена Сергеевна} and Emma Evergren and Volker Haucke and Lennart Brodin and Шупляков, {Олег Викторович}",
year = "2019",
language = "русский",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",

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Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation : Synaptic vesicle clustering in a liquid phase. / Pechstein, Arndt; Tomilin, Nikolay; Walther, Kristin; Vorontsova, Olga; Сопова, Елена Сергеевна; Evergren, Emma; Haucke, Volker; Brodin, Lennart; Шупляков, Олег Викторович.

В: Cell Reports, 2019.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation

T2 - Synaptic vesicle clustering in a liquid phase

AU - Pechstein, Arndt

AU - Tomilin, Nikolay

AU - Walther, Kristin

AU - Vorontsova, Olga

AU - Сопова, Елена Сергеевна

AU - Evergren, Emma

AU - Haucke, Volker

AU - Brodin, Lennart

AU - Шупляков, Олег Викторович

PY - 2019

Y1 - 2019

N2 - Liquid-liquid phase separation is an increasingly recognized mechanism for compartmentalization in cells. Recent in vitro studies suggest that this organizational principle may apply to synaptic vesicle clusters. Here we test this possibility by performing microinjections at the living lamprey giant reticulospinal synapse. Axons were maintained at rest to examine if reagents introduced into the cytosol would enter a putative liquid phase to disrupt critical protein-protein interactions. We found that compounds that perturb the intrinsically disordered region of synapsin, which is critical for liquid phase organization in vitro, cause dispersion of synaptic vesicles from resting clusters. Reagents that perturb SH3 domain interactions with synapsin were found ineffective at rest. Our results indicate that synaptic vesicles at a living central synapse are organized as a distinct liquid phase maintained by interactions via the intrinsically disordered region of synapsin.

AB - Liquid-liquid phase separation is an increasingly recognized mechanism for compartmentalization in cells. Recent in vitro studies suggest that this organizational principle may apply to synaptic vesicle clusters. Here we test this possibility by performing microinjections at the living lamprey giant reticulospinal synapse. Axons were maintained at rest to examine if reagents introduced into the cytosol would enter a putative liquid phase to disrupt critical protein-protein interactions. We found that compounds that perturb the intrinsically disordered region of synapsin, which is critical for liquid phase organization in vitro, cause dispersion of synaptic vesicles from resting clusters. Reagents that perturb SH3 domain interactions with synapsin were found ineffective at rest. Our results indicate that synaptic vesicles at a living central synapse are organized as a distinct liquid phase maintained by interactions via the intrinsically disordered region of synapsin.

KW - synapse

KW - synaptic vesicle

KW - synapsin

KW - accessory proteins

KW - phase separation

KW - SH3-domain interaction

M3 - статья

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

ER -