Transdifferentiation and mesenchymal-to-epithelial transition during regeneration in Demospongiae (Porifera)

Alexander V. Ereskovsky, Daria B. Tokina, Danial M. Saidov, Stephen Baghdiguian, Emilie Le Goff, Andrey I. Lavrov

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Origin and early evolution of regeneration mechanisms remain among the most pressing questions in animal regeneration biology. Porifera have exceptional regenerative capacities and, as early Metazoan lineage, are a promising model for studying evolutionary aspects of regeneration. Here, we focus on reparative regeneration of the body wall in the Mediterranean demosponge Aplysina cavernicola. The epithelialization of the wound surface is completed within 2 days, and the wound is completely healed within 2 weeks. The regeneration is accompanied with the formation of a mass of undifferentiated cells (blastema), which consists of archaeocytes, dedifferentiated choanocytes, anucleated amoebocytes, and differentiated spherulous cells. The main mechanisms of A. cavernicola regeneration are cell dedifferentiation with active migration and subsequent redifferentiation or transdifferentiation of polypotent cells through the mesenchymal‐to‐epithelial transformation. The main cell sources of the regeneration are archaeocytes and choanocytes. At early stages of the regeneration, the blastema almost devoid of cell proliferation, but after 24 hr postoperation (hpo) and up to 72 hpo numerous DNA‐synthesizing cells appear there. In contrast to intact tissues, where vast majority of DNA‐synthesizing cells are choanocytes, all 5‐ethynyl‐2′‐deoxyuridine‐labeled cells in the blastema are mesohyl cells. Intact tissues, distant from the wound, retains intact level of cell proliferation during whole regeneration process. For the first time, the apoptosis was studied during the regeneration of sponges. Two waves of apoptosis were detected during A. cavernicola regeneration: The first wave at 6–12 hpo and the second wave at 48–72 hpo.
Язык оригиналаанглийский
ЖурналJournal of Experimental Zoology Part B: Molecular and Developmental Evolution
DOI
СостояниеЭлектронная публикация перед печатью - 14 ноя 2019

Отпечаток

Epithelial-Mesenchymal Transition
Porifera
Regeneration
regeneration
Cavernicola
animal injuries
cells
cell proliferation
apoptosis
cell dedifferentiation
Demospongiae
Cell Transdifferentiation
Cell Dedifferentiation
Cell Proliferation
Apoptosis
Re-Epithelialization
integument
metazoan
Wounds and Injuries
sponge

Предметные области Scopus

  • Земледелие и биологические науки (все)

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title = "Transdifferentiation and mesenchymal-to-epithelial transition during regeneration in Demospongiae (Porifera)",
abstract = "Origin and early evolution of regeneration mechanisms remain among the most pressing questions in animal regeneration biology. Porifera have exceptional regenerative capacities and, as early Metazoan lineage, are a promising model for studying evolutionary aspects of regeneration. Here, we focus on reparative regeneration of the body wall in the Mediterranean demosponge Aplysina cavernicola. The epithelialization of the wound surface is completed within 2 days, and the wound is completely healed within 2 weeks. The regeneration is accompanied with the formation of a mass of undifferentiated cells (blastema), which consists of archaeocytes, dedifferentiated choanocytes, anucleated amoebocytes, and differentiated spherulous cells. The main mechanisms of A. cavernicola regeneration are cell dedifferentiation with active migration and subsequent redifferentiation or transdifferentiation of polypotent cells through the mesenchymal‐to‐epithelial transformation. The main cell sources of the regeneration are archaeocytes and choanocytes. At early stages of the regeneration, the blastema almost devoid of cell proliferation, but after 24 hr postoperation (hpo) and up to 72 hpo numerous DNA‐synthesizing cells appear there. In contrast to intact tissues, where vast majority of DNA‐synthesizing cells are choanocytes, all 5‐ethynyl‐2′‐deoxyuridine‐labeled cells in the blastema are mesohyl cells. Intact tissues, distant from the wound, retains intact level of cell proliferation during whole regeneration process. For the first time, the apoptosis was studied during the regeneration of sponges. Two waves of apoptosis were detected during A. cavernicola regeneration: The first wave at 6–12 hpo and the second wave at 48–72 hpo.",
keywords = "apoptosis, blastema, demosponges, mesenchymal‐to‐epithelial transformation, Regeneration, Transdifferentiation",
author = "Ereskovsky, {Alexander V.} and Tokina, {Daria B.} and Saidov, {Danial M.} and Stephen Baghdiguian and {Le Goff}, Emilie and Lavrov, {Andrey I.}",
year = "2019",
month = "11",
day = "14",
doi = "10.1002/jez.b.22919",
language = "English",
journal = "Journal of Experimental Zoology Part B: Molecular and Developmental Evolution",
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Transdifferentiation and mesenchymal-to-epithelial transition during regeneration in Demospongiae (Porifera). / Ereskovsky, Alexander V. ; Tokina, Daria B. ; Saidov, Danial M. ; Baghdiguian, Stephen; Le Goff, Emilie; Lavrov, Andrey I. .

В: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 14.11.2019.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Transdifferentiation and mesenchymal-to-epithelial transition during regeneration in Demospongiae (Porifera)

AU - Ereskovsky, Alexander V.

AU - Tokina, Daria B.

AU - Saidov, Danial M.

AU - Baghdiguian, Stephen

AU - Le Goff, Emilie

AU - Lavrov, Andrey I.

PY - 2019/11/14

Y1 - 2019/11/14

N2 - Origin and early evolution of regeneration mechanisms remain among the most pressing questions in animal regeneration biology. Porifera have exceptional regenerative capacities and, as early Metazoan lineage, are a promising model for studying evolutionary aspects of regeneration. Here, we focus on reparative regeneration of the body wall in the Mediterranean demosponge Aplysina cavernicola. The epithelialization of the wound surface is completed within 2 days, and the wound is completely healed within 2 weeks. The regeneration is accompanied with the formation of a mass of undifferentiated cells (blastema), which consists of archaeocytes, dedifferentiated choanocytes, anucleated amoebocytes, and differentiated spherulous cells. The main mechanisms of A. cavernicola regeneration are cell dedifferentiation with active migration and subsequent redifferentiation or transdifferentiation of polypotent cells through the mesenchymal‐to‐epithelial transformation. The main cell sources of the regeneration are archaeocytes and choanocytes. At early stages of the regeneration, the blastema almost devoid of cell proliferation, but after 24 hr postoperation (hpo) and up to 72 hpo numerous DNA‐synthesizing cells appear there. In contrast to intact tissues, where vast majority of DNA‐synthesizing cells are choanocytes, all 5‐ethynyl‐2′‐deoxyuridine‐labeled cells in the blastema are mesohyl cells. Intact tissues, distant from the wound, retains intact level of cell proliferation during whole regeneration process. For the first time, the apoptosis was studied during the regeneration of sponges. Two waves of apoptosis were detected during A. cavernicola regeneration: The first wave at 6–12 hpo and the second wave at 48–72 hpo.

AB - Origin and early evolution of regeneration mechanisms remain among the most pressing questions in animal regeneration biology. Porifera have exceptional regenerative capacities and, as early Metazoan lineage, are a promising model for studying evolutionary aspects of regeneration. Here, we focus on reparative regeneration of the body wall in the Mediterranean demosponge Aplysina cavernicola. The epithelialization of the wound surface is completed within 2 days, and the wound is completely healed within 2 weeks. The regeneration is accompanied with the formation of a mass of undifferentiated cells (blastema), which consists of archaeocytes, dedifferentiated choanocytes, anucleated amoebocytes, and differentiated spherulous cells. The main mechanisms of A. cavernicola regeneration are cell dedifferentiation with active migration and subsequent redifferentiation or transdifferentiation of polypotent cells through the mesenchymal‐to‐epithelial transformation. The main cell sources of the regeneration are archaeocytes and choanocytes. At early stages of the regeneration, the blastema almost devoid of cell proliferation, but after 24 hr postoperation (hpo) and up to 72 hpo numerous DNA‐synthesizing cells appear there. In contrast to intact tissues, where vast majority of DNA‐synthesizing cells are choanocytes, all 5‐ethynyl‐2′‐deoxyuridine‐labeled cells in the blastema are mesohyl cells. Intact tissues, distant from the wound, retains intact level of cell proliferation during whole regeneration process. For the first time, the apoptosis was studied during the regeneration of sponges. Two waves of apoptosis were detected during A. cavernicola regeneration: The first wave at 6–12 hpo and the second wave at 48–72 hpo.

KW - apoptosis

KW - blastema

KW - demosponges

KW - mesenchymal‐to‐epithelial transformation

KW - Regeneration

KW - Transdifferentiation

U2 - 10.1002/jez.b.22919

DO - 10.1002/jez.b.22919

M3 - Article

JO - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

JF - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

SN - 1552-5007

ER -