Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines

A.A. Tkachenko, M.S. Gancheva, V.E. Tvorogova, L.G. Danilov, A.V. Predeus, I.E. Dodueva, L.A. Lutova

Результат исследований: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Studying aberrations of normal developmental processes like plant tumor initiation provides a great opportunity for understanding of systemic control of plant cells differentiation. Most well‐studied example of plant tumor is the crown gall caused by Agrobacterium tumefaciens; much less studied are spontaneous tumors developing in plants with certain genotype, regardless of agrobacterial infection. In the present study, we used RNA‐seq to analyze the changes in gene expression at the early stages of development of A. tumefaciens‐induced tumors in radish seedlings. In our studies we used two closely related radish inbred lines contrasting in their ability to form spontaneous tumors. RNA‐seq and qPCR analysis showed significant changes in the expression levels for numerous genes involved in different metabolic and developmental processes, and Gene Ontology analysis indicated many biological processes probably involved in the crown gall development in radish. For example, a unique result of our research is upregulation of genes related to photomorphogenesis and downregulation of senescence‐associated genes in the young crown gall tumors. Differences in gene expression between the analyzed radish lines included upregulation of pathways related to chromatin remodeling and protein targeting to chloroplast in the tumorous line along with downregulation of pathways of chaperone‐mediated complex assembly and anthocyanin biosynthesis.
Язык оригиналаанглийский
ЖурналAnnals of Applied Biology
Ранняя дата в режиме онлайн2 янв 2021
СостояниеЭлектронная публикация перед печатью - 2 янв 2021

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