The dynamin-binding domains of Dap160/intersectin affect bulk membrane retrieval in synapses

Åsa M.E. Winther, Wei Jiao, Olga Vorontsova, Kathryn A. Rees, Tong Wey Koh, Elena Sopova, Karen L. Schulze, Hugo J. Bellen, Oleg Shupliakov

Результат исследований: Научные публикации в периодических изданияхстатья

17 Цитирования (Scopus)


Dynamin-associated protein 160 kDa (Dap160)/intersectin interacts with several synaptic proteins and affects endocytosis and synapse development. The functional role of the different protein interaction domains is not well understood. Here we show that Drosophila Dap160 lacking the dynamin-binding SH3 domains does not affect the development of the neuromuscular junction but plays a key role in synaptic vesicle recycling. dap160 mutants lacking dynamin-interacting domains no longer accumulate dynamin properly at the periactive zone, and it becomes dispersed in the bouton during stimulation. This is accompanied by a reduction in uptake of the dye FM1-43 and an accumulation of large vesicles and membrane invaginations. However, we do not observe an increase in the number of clathrin-coated intermediates. We also note a depression in evoked excitatory junction potentials (EJPs) during high-rate stimulation, accompanied by aberrantly large miniature EJPs. The data reveal the important role of Dap160 in the targeting of dynamin to the periactive zone, where it is required to suppress bulk synaptic vesicle membrane retrieval during high-frequency activity.

Язык оригиналаанглийский
Страницы (с-по)1021-1031
Число страниц11
ЖурналJournal of Cell Science
Номер выпуска4
СостояниеОпубликовано - 15 фев 2013

Предметные области Scopus

  • Клеточная биология

Fingerprint Подробные сведения о темах исследования «The dynamin-binding domains of Dap160/intersectin affect bulk membrane retrieval in synapses». Вместе они формируют уникальный семантический отпечаток (fingerprint).