Reduction of PII signaling protein enhances lipid body production in Chlamydomonas reinhardtii

Z. Zalutskaya, N. Kharatyan, K. Forchhammer, E. Ermilova

Результат исследований: Научные публикации в периодических изданияхстатья

19 Цитирования (Scopus)

Аннотация

In all examined organisms that have the PII signal transduction machinery, PII coordinates the central C/N anabolic metabolism. In green algae and land plants, PII is localized in the chloroplast and controls the L-arginine biosynthetic pathway pathway. To elucidate additional functions of PII in the model photosynthetic organism Chlamydomonas reinhardtii (CrPII), we generated and analyzed four strains, in which PII was strongly under-expressed by artificial microRNA (GLB1-amiRNA strains). In response to nitrogen deficiency, Chlamydomonas produces triacylglycerols (TAGs) that are accumulated in lipid bodies (LB). Quantification of LBs by confocal microscopy in four GLB1-amiRNA strains showed that reduced PII levels resulted in over-accumulation of LBs compared to their parental strains. Moreover, knock-down of PII caused also an increase in the total TAG level. We propose that the larger yields of TAG-filled LBs in N-starved GLB1-amiRNA cells can be attributed to the strain's depleted PII level and their inab
Язык оригиналаанглийский
Страницы (с-по)1-9
ЖурналPlant Science
Том240
DOI
СостояниеОпубликовано - 2015

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