Proteomic Profiling of the Human Fetal Multipotent Mesenchymal Stromal Cells Secretome

Arseniy A. Lobov, Natalia M. Yudintceva, Alexey G. Mittenberg, Sergey V. Shabelnikov, Natalia A. Mikhailova, Anna B. Malashicheva, Mikhail G. Khotin

Результат исследований: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Secretome of multipotent mesenchymal stromal cells (MSCs) is actively used in biomedical applications such as alveolar bone regeneration, treatment of cardiovascular disease, and neurodegenerative disorders. Nevertheless, hMSCs have low proliferative potential and production of the industrial quantity of their secretome might be challenging. Human fetal multipotent mesenchymal stromal cells (FetMSCs) isolated from early human embryo bone marrow are easy to expand and might be a potential source for pharmaceutical substances production based on their secretome. However, the secretome of FetMSCs was not previously analyzed. Here, we describe the secretome of FetMSCs using LC-MALDI shotgun proteomics. We identified 236 proteins. Functional annotation of the identified proteins revealed their involvement in angiogenesis, ossification, regulation of apoptosis, and immune response processes, which made it promising for biomedical applications. The proteins identified in the FetMSCs secretome are involved in the same biological processes as proteins from previously described adult hMSCs secretomes. Nevertheless, many of the common hMSCs secretome components (such as VEGF, FGF, Wnt and TGF-β) have not been identified in the FetMSCs secretome.

Язык оригиналаанглийский
ЖурналMolecules (Basel, Switzerland)
Том25
Номер выпуска22
DOI
СостояниеОпубликовано - 12 ноя 2020
Опубликовано для внешнего пользованияДа

Предметные области Scopus

  • Аналитическая химия
  • Химия (разное)
  • Молекулярная медицина
  • Фармация
  • Поиск новых лекарств
  • Физическая и теоретическая химия
  • Органическая химия

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