Proteome Map of Pea (Pisum sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

Tatiana Mamontova, Elena Lukasheva, Gregory Mavropolo-Stolyarenko, Carsten Proksch, Tatiana Bilova, Ahyoung Kim, Vladimir Babakov, Tatiana Grishina, Wolfgang Hoehenwarter, Sergei Medvedev, Galina Smolikova, Andrej Frolov

Результат исследований: Научные публикации в периодических изданияхстатьярецензирование

7 Цитирования (Scopus)

Аннотация

Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Apparently, changes in seed protein patterns might directly affect both of these aspects. Thus, here, we address the pea seed proteome in detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. To address possible intercultivar differences, we compared seed proteomes of yellow- and green-seeded pea cultivars in a comprehensive case study. The analysis revealed totally 1938 and 1989 nonredundant proteins, respectively. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP), potentially indicating the high efficiency of our experimental workflow. Totally 981 protein groups were assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.
Язык оригиналаанглийский
Номер статьи4066
Страницы (с-по)4066
ЖурналInternational Journal of Molecular Sciences
Том19
Номер выпуска12
DOI
СостояниеОпубликовано - 15 дек 2018

Предметные области Scopus

  • Биохимия, генетика и молекулярная биология (все)
  • Молекулярная биология
  • Спектроскопия
  • Катализ
  • Неорганическая химия
  • Прикладные компьютерные науки
  • Физическая и теоретическая химия
  • Органическая химия

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