Proteome dynamics of antibiotic resistant Staphylococcus aureus strains exposed to sub-inhibitory concentrations of beta-lactams

Y. V. Sopova, V. V. Gostev, O. S. Kalinogorskaya, A. N. Lykholay, S. V. Sidorenko

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

Выдержка

Background. Ceftaroline is one of the first cephalosporins with activity against methicillin-resistant Staphylococcus aureus (MRSA), it effectively binds to and inhibits penicillin-binding protein 2a (PBP2a). However, isolates with decreased susceptibility to ceftaroline were reported before the commercial release of the antibiotic. The aim of this study was to provide an overview of the proteome changes occurring in MRSA isolates resistant to ceftaroline in response to sub-inhibitory concentrations of cell-wall active antibiotics. Materials and methods. Ceftaroline-resistant mutants were generated from two MRSA SA0077 and SA0422 isolates belonging to ST8-T008-SCCmec IV genetic lineage (sequence type 8, spa type t008, staphylococcal chromosomal cassette mec type IV) and one MRSA isolate SA0085 belonging to ST239-T631-SCCmec III genetic lineage (sequence type 239, spa type t631, staphylococcal chromosomal cassette mec type III). Proteome response of parental and mutant strains to subinhibitory concentration of beta-lactams and vancomycin was analyzed. Results. The protein patterns revealed significant increase of 30 κDa band in mutant strains under induction by meropenem, no changes were observed in parental strains or under induction with other antibiotics. According to MS analysis, three proteins represented the band of the mutant strain in absence of meropenem induction. However, under meropenem induction additional protein was detected (BlaZ). Conclusion. The cross talk between two systems with overlapping functions involved in transcription control of PBP2a and BlaZ ensure ceftaroline resistant phenotype.

Язык оригиналарусский
Страницы (с-по)4-10
Число страниц7
ЖурналEcological Genetics
Том16
Номер выпуска2
DOI
СостояниеОпубликовано - 2018

Ключевые слова

    Предметные области Scopus

    • Экология, эволюция поведение и систематика
    • Биохимия
    • Экология
    • Генетика
    • Генетика (клиническая)

    Цитировать

    Sopova, Y. V. ; Gostev, V. V. ; Kalinogorskaya, O. S. ; Lykholay, A. N. ; Sidorenko, S. V. / Proteome dynamics of antibiotic resistant Staphylococcus aureus strains exposed to sub-inhibitory concentrations of beta-lactams. В: Ecological Genetics. 2018 ; Том 16, № 2. стр. 4-10.
    @article{d82bf59900064161971ec6dc6855d482,
    title = "Proteome dynamics of antibiotic resistant Staphylococcus aureus strains exposed to sub-inhibitory concentrations of beta-lactams",
    abstract = "Background. Ceftaroline is one of the first cephalosporins with activity against methicillin-resistant Staphylococcus aureus (MRSA), it effectively binds to and inhibits penicillin-binding protein 2a (PBP2a). However, isolates with decreased susceptibility to ceftaroline were reported before the commercial release of the antibiotic. The aim of this study was to provide an overview of the proteome changes occurring in MRSA isolates resistant to ceftaroline in response to sub-inhibitory concentrations of cell-wall active antibiotics. Materials and methods. Ceftaroline-resistant mutants were generated from two MRSA SA0077 and SA0422 isolates belonging to ST8-T008-SCCmec IV genetic lineage (sequence type 8, spa type t008, staphylococcal chromosomal cassette mec type IV) and one MRSA isolate SA0085 belonging to ST239-T631-SCCmec III genetic lineage (sequence type 239, spa type t631, staphylococcal chromosomal cassette mec type III). Proteome response of parental and mutant strains to subinhibitory concentration of beta-lactams and vancomycin was analyzed. Results. The protein patterns revealed significant increase of 30 κDa band in mutant strains under induction by meropenem, no changes were observed in parental strains or under induction with other antibiotics. According to MS analysis, three proteins represented the band of the mutant strain in absence of meropenem induction. However, under meropenem induction additional protein was detected (BlaZ). Conclusion. The cross talk between two systems with overlapping functions involved in transcription control of PBP2a and BlaZ ensure ceftaroline resistant phenotype.",
    keywords = "Antibiotics, MRSA., Proteome",
    author = "Sopova, {Y. V.} and Gostev, {V. V.} and Kalinogorskaya, {O. S.} and Lykholay, {A. N.} and Sidorenko, {S. V.}",
    year = "2018",
    doi = "10.17816/ecogen1624-10",
    language = "русский",
    volume = "16",
    pages = "4--10",
    journal = "ЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА",
    issn = "1811-0932",
    publisher = "Эко-Вектор",
    number = "2",

    }

    Proteome dynamics of antibiotic resistant Staphylococcus aureus strains exposed to sub-inhibitory concentrations of beta-lactams. / Sopova, Y. V.; Gostev, V. V.; Kalinogorskaya, O. S.; Lykholay, A. N.; Sidorenko, S. V.

    В: Ecological Genetics, Том 16, № 2, 2018, стр. 4-10.

    Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

    TY - JOUR

    T1 - Proteome dynamics of antibiotic resistant Staphylococcus aureus strains exposed to sub-inhibitory concentrations of beta-lactams

    AU - Sopova, Y. V.

    AU - Gostev, V. V.

    AU - Kalinogorskaya, O. S.

    AU - Lykholay, A. N.

    AU - Sidorenko, S. V.

    PY - 2018

    Y1 - 2018

    N2 - Background. Ceftaroline is one of the first cephalosporins with activity against methicillin-resistant Staphylococcus aureus (MRSA), it effectively binds to and inhibits penicillin-binding protein 2a (PBP2a). However, isolates with decreased susceptibility to ceftaroline were reported before the commercial release of the antibiotic. The aim of this study was to provide an overview of the proteome changes occurring in MRSA isolates resistant to ceftaroline in response to sub-inhibitory concentrations of cell-wall active antibiotics. Materials and methods. Ceftaroline-resistant mutants were generated from two MRSA SA0077 and SA0422 isolates belonging to ST8-T008-SCCmec IV genetic lineage (sequence type 8, spa type t008, staphylococcal chromosomal cassette mec type IV) and one MRSA isolate SA0085 belonging to ST239-T631-SCCmec III genetic lineage (sequence type 239, spa type t631, staphylococcal chromosomal cassette mec type III). Proteome response of parental and mutant strains to subinhibitory concentration of beta-lactams and vancomycin was analyzed. Results. The protein patterns revealed significant increase of 30 κDa band in mutant strains under induction by meropenem, no changes were observed in parental strains or under induction with other antibiotics. According to MS analysis, three proteins represented the band of the mutant strain in absence of meropenem induction. However, under meropenem induction additional protein was detected (BlaZ). Conclusion. The cross talk between two systems with overlapping functions involved in transcription control of PBP2a and BlaZ ensure ceftaroline resistant phenotype.

    AB - Background. Ceftaroline is one of the first cephalosporins with activity against methicillin-resistant Staphylococcus aureus (MRSA), it effectively binds to and inhibits penicillin-binding protein 2a (PBP2a). However, isolates with decreased susceptibility to ceftaroline were reported before the commercial release of the antibiotic. The aim of this study was to provide an overview of the proteome changes occurring in MRSA isolates resistant to ceftaroline in response to sub-inhibitory concentrations of cell-wall active antibiotics. Materials and methods. Ceftaroline-resistant mutants were generated from two MRSA SA0077 and SA0422 isolates belonging to ST8-T008-SCCmec IV genetic lineage (sequence type 8, spa type t008, staphylococcal chromosomal cassette mec type IV) and one MRSA isolate SA0085 belonging to ST239-T631-SCCmec III genetic lineage (sequence type 239, spa type t631, staphylococcal chromosomal cassette mec type III). Proteome response of parental and mutant strains to subinhibitory concentration of beta-lactams and vancomycin was analyzed. Results. The protein patterns revealed significant increase of 30 κDa band in mutant strains under induction by meropenem, no changes were observed in parental strains or under induction with other antibiotics. According to MS analysis, three proteins represented the band of the mutant strain in absence of meropenem induction. However, under meropenem induction additional protein was detected (BlaZ). Conclusion. The cross talk between two systems with overlapping functions involved in transcription control of PBP2a and BlaZ ensure ceftaroline resistant phenotype.

    KW - Antibiotics

    KW - MRSA.

    KW - Proteome

    UR - http://www.scopus.com/inward/record.url?scp=85052833263&partnerID=8YFLogxK

    U2 - 10.17816/ecogen1624-10

    DO - 10.17816/ecogen1624-10

    M3 - статья

    VL - 16

    SP - 4

    EP - 10

    JO - ЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА

    JF - ЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА

    SN - 1811-0932

    IS - 2

    ER -