Polymer particles bearing recombinant lel cd81 as trapping systems for hepatitis c virus

Dmitry Polyakov, Ekaterina Sinitsyna, Natalia Grudinina, Mariia Antipchik, Rodion Sakhabeev, Viktor Korzhikov-Vlakh, Mikhail Shavlovsky, Evgenia Korzhikova-Vlakh, Tatiana Tennikova

Результат исследований: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro-and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro-and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro-and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.

Язык оригиналаанглийский
Номер статьи672
ЖурналPharmaceutics
Том13
Номер выпуска5
DOI
СостояниеОпубликовано - мая 2021

Предметные области Scopus

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