Placental microRNA expression in pregnancies complicated by superimposed pre-eclampsia on chronic hypertension

Elena S. Vashukova, Andrey S. Glotov, Pavel V. Fedotov, Olga A. Efimova, Vladimir S. Pakin, Elena V. Mozgovaya, Anna A. Pendina, Andrei V. Tikhonov, Alla S. Koltsova, Vladislav S. Baranov

    Результат исследований: Научные публикации в периодических изданияхстатья

    18 Цитирования (Scopus)

    Выдержка

    Pre-eclampsia (PE) is a complication of pregnancy that affects 5-8% of women after 20 weeks of gestation. It is usually diagnosed based on the de novo onset of hypertension and proteinuria. Preexisting hypertension in women developing PE, also known as superimposed PE on chronic hypertension (SPE), leads to elevated risk of maternal and fetal mortality. PE is associated with an altered microRNA (miRNA) expression pattern in the placenta, suggesting that miRNA deregulation is involved in the pathogenesis of PE. Whether and how the miRNA expression pattern is changed in the SPE placenta remains unclear. The present study analyzed the placental miRNA expression profile in pregnancies complicated by SPE. miRNA expression profiles in SPE and normal placentas were investigated using an Ion Torrent sequencing system. Sequencing data were processed using a comprehensive analysis pipeline for deep miRNA sequencing (CAP-miRSeq). A total of 22 miRNAs were identified to be deregulated in placentas from patients with SPE. They included 16 miRNAs previously known to be associated with PE and 6 novel miRNAs. Among the 6 novel miRNAs, 4 were upregulated (miR-518a, miR-527, miR-518e and miR-4532) and 2 downregulated (miR-98 and miR-135b) in SPE placentas compared with controls. The present results suggest that SPE is associated with specific alterations in the placental miRNA expression pattern, which differ from alterations detected in PE placentas, and therefore, provide novel targets for further investigation of the molecular mechanisms underlying SPE pathogenesis.

    Язык оригиналаанглийский
    Страницы (с-по)22-32
    Число страниц11
    ЖурналMolecular Medicine Reports
    Том14
    Номер выпуска1
    DOI
    СостояниеОпубликовано - 1 июл 2016

    Отпечаток

    Pre-Eclampsia
    MicroRNAs
    Hypertension
    Pregnancy
    Placenta
    Fetal Mortality
    High-Throughput Nucleotide Sequencing
    Deregulation
    Pregnancy Complications
    Maternal Mortality
    Proteinuria
    Down-Regulation
    Pipelines
    Ions

    Предметные области Scopus

    • Биохимия
    • Молекулярная медицина
    • Молекулярная биология
    • Генетика
    • Онкология
    • Исследования рака

    Цитировать

    Vashukova, Elena S. ; Glotov, Andrey S. ; Fedotov, Pavel V. ; Efimova, Olga A. ; Pakin, Vladimir S. ; Mozgovaya, Elena V. ; Pendina, Anna A. ; Tikhonov, Andrei V. ; Koltsova, Alla S. ; Baranov, Vladislav S. / Placental microRNA expression in pregnancies complicated by superimposed pre-eclampsia on chronic hypertension. В: Molecular Medicine Reports. 2016 ; Том 14, № 1. стр. 22-32.
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    title = "Placental microRNA expression in pregnancies complicated by superimposed pre-eclampsia on chronic hypertension",
    abstract = "Pre-eclampsia (PE) is a complication of pregnancy that affects 5-8{\%} of women after 20 weeks of gestation. It is usually diagnosed based on the de novo onset of hypertension and proteinuria. Preexisting hypertension in women developing PE, also known as superimposed PE on chronic hypertension (SPE), leads to elevated risk of maternal and fetal mortality. PE is associated with an altered microRNA (miRNA) expression pattern in the placenta, suggesting that miRNA deregulation is involved in the pathogenesis of PE. Whether and how the miRNA expression pattern is changed in the SPE placenta remains unclear. The present study analyzed the placental miRNA expression profile in pregnancies complicated by SPE. miRNA expression profiles in SPE and normal placentas were investigated using an Ion Torrent sequencing system. Sequencing data were processed using a comprehensive analysis pipeline for deep miRNA sequencing (CAP-miRSeq). A total of 22 miRNAs were identified to be deregulated in placentas from patients with SPE. They included 16 miRNAs previously known to be associated with PE and 6 novel miRNAs. Among the 6 novel miRNAs, 4 were upregulated (miR-518a, miR-527, miR-518e and miR-4532) and 2 downregulated (miR-98 and miR-135b) in SPE placentas compared with controls. The present results suggest that SPE is associated with specific alterations in the placental miRNA expression pattern, which differ from alterations detected in PE placentas, and therefore, provide novel targets for further investigation of the molecular mechanisms underlying SPE pathogenesis.",
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    author = "Vashukova, {Elena S.} and Glotov, {Andrey S.} and Fedotov, {Pavel V.} and Efimova, {Olga A.} and Pakin, {Vladimir S.} and Mozgovaya, {Elena V.} and Pendina, {Anna A.} and Tikhonov, {Andrei V.} and Koltsova, {Alla S.} and Baranov, {Vladislav S.}",
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    Vashukova, ES, Glotov, AS, Fedotov, PV, Efimova, OA, Pakin, VS, Mozgovaya, EV, Pendina, AA, Tikhonov, AV, Koltsova, AS & Baranov, VS 2016, 'Placental microRNA expression in pregnancies complicated by superimposed pre-eclampsia on chronic hypertension', Molecular Medicine Reports, том. 14, № 1, стр. 22-32. https://doi.org/10.3892/mmr.2016.5268

    Placental microRNA expression in pregnancies complicated by superimposed pre-eclampsia on chronic hypertension. / Vashukova, Elena S.; Glotov, Andrey S.; Fedotov, Pavel V.; Efimova, Olga A.; Pakin, Vladimir S.; Mozgovaya, Elena V.; Pendina, Anna A.; Tikhonov, Andrei V.; Koltsova, Alla S.; Baranov, Vladislav S.

    В: Molecular Medicine Reports, Том 14, № 1, 01.07.2016, стр. 22-32.

    Результат исследований: Научные публикации в периодических изданияхстатья

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    AU - Vashukova, Elena S.

    AU - Glotov, Andrey S.

    AU - Fedotov, Pavel V.

    AU - Efimova, Olga A.

    AU - Pakin, Vladimir S.

    AU - Mozgovaya, Elena V.

    AU - Pendina, Anna A.

    AU - Tikhonov, Andrei V.

    AU - Koltsova, Alla S.

    AU - Baranov, Vladislav S.

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