Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes

Sergei Medvedev, Olga Voronina, Olga Tankelyun, Tatiana Bilova, Dmitry Suslov, Mikhail Bankin, Viera Mackievic, Maryia Makavitskaya, Maria Shishova, Jan Martinec, Galina Smolikova, Elena Sharova, Vadim Demidchik

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Phosphatidic acids (PAs) are a key intermediate in phospholipid biosynthesis, and a central element in numerous signalling pathways. Functions of PAs are related to their fundamental role in molecular interactions within cell membranes modifying membrane bending, budding, fission and fusion. Here we tested the hypothesis that PAs are capable of direct transport of ions across bio-membranes. We have demonstrated that PAs added to the maize plasma membrane vesicles induced ionophore-like transmembrane transport of Ca 2+ , H + and Mg 2+ . PA-induced Ca 2+ fluxes increased with an increasing PAs acyl chain unsaturation. For all the PAs analysed, the effect on Ca 2+ permeability increased with increasing pH (pH 8.0 > pH 7.2 > pH 6.0). The PA-induced Ca 2+ , Mg 2+ and H + permeability was also more pronounced in the endomembrane vesicles as compared with the plasma membrane vesicles. Addition of PA to protoplasts from Arabidopsis thaliana (L.) Heynh. roots constitutively expressing aequorin triggered elevation of the cytosolic Ca 2+ activity, indicating that the observed PA-dependent Ca 2+ transport occurs in intact plants.

Язык оригиналаанглийский
Страницы (с-по)533-542
Число страниц10
ЖурналFunctional Plant Biology
Том46
Номер выпуска6
Ранняя дата в режиме онлайн1 янв 2019
DOI
СостояниеОпубликовано - 2019

Отпечаток

cell membranes
acids
permeability
plasma membrane
ionophores
ion transport
protoplasts
phospholipids
Arabidopsis thaliana
biosynthesis
corn

Предметные области Scopus

  • Агрономия и растениеводство
  • Прикладная ботаника

Цитировать

Medvedev, Sergei ; Voronina, Olga ; Tankelyun, Olga ; Bilova, Tatiana ; Suslov, Dmitry ; Bankin, Mikhail ; Mackievic, Viera ; Makavitskaya, Maryia ; Shishova, Maria ; Martinec, Jan ; Smolikova, Galina ; Sharova, Elena ; Demidchik, Vadim. / Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes. В: Functional Plant Biology. 2019 ; Том 46, № 6. стр. 533-542.
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title = "Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes",
abstract = "Phosphatidic acids (PAs) are a key intermediate in phospholipid biosynthesis, and a central element in numerous signalling pathways. Functions of PAs are related to their fundamental role in molecular interactions within cell membranes modifying membrane bending, budding, fission and fusion. Here we tested the hypothesis that PAs are capable of direct transport of ions across bio-membranes. We have demonstrated that PAs added to the maize plasma membrane vesicles induced ionophore-like transmembrane transport of Ca 2+ , H + and Mg 2+ . PA-induced Ca 2+ fluxes increased with an increasing PAs acyl chain unsaturation. For all the PAs analysed, the effect on Ca 2+ permeability increased with increasing pH (pH 8.0 > pH 7.2 > pH 6.0). The PA-induced Ca 2+ , Mg 2+ and H + permeability was also more pronounced in the endomembrane vesicles as compared with the plasma membrane vesicles. Addition of PA to protoplasts from Arabidopsis thaliana (L.) Heynh. roots constitutively expressing aequorin triggered elevation of the cytosolic Ca 2+ activity, indicating that the observed PA-dependent Ca 2+ transport occurs in intact plants.",
keywords = "Ca signaling, Ca transport, H transport, membrane vesicles, phosphatidic acids, protoplasts, Ca 2+ transport, H + transport, Ca 2+ signaling, Ca2+ signaling, PHOSPHOLIPASE-D, CHLORTETRACYCLINE, ION, MECHANISMS, PLASMA-MEMBRANE, MAGNESIUM, DIVALENT-CATIONS, CALCIUM, STRESS, H+ transport, Ca2+ transport, NONSELECTIVE CATION CHANNELS",
author = "Sergei Medvedev and Olga Voronina and Olga Tankelyun and Tatiana Bilova and Dmitry Suslov and Mikhail Bankin and Viera Mackievic and Maryia Makavitskaya and Maria Shishova and Jan Martinec and Galina Smolikova and Elena Sharova and Vadim Demidchik",
year = "2019",
doi = "10.1071/FP18242",
language = "English",
volume = "46",
pages = "533--542",
journal = "Functional Plant Biology",
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Medvedev, S, Voronina, O, Tankelyun, O, Bilova, T, Suslov, D, Bankin, M, Mackievic, V, Makavitskaya, M, Shishova, M, Martinec, J, Smolikova, G, Sharova, E & Demidchik, V 2019, 'Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes', Functional Plant Biology, том. 46, № 6, стр. 533-542. https://doi.org/10.1071/FP18242

Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes. / Medvedev, Sergei; Voronina, Olga; Tankelyun, Olga; Bilova, Tatiana; Suslov, Dmitry; Bankin, Mikhail; Mackievic, Viera; Makavitskaya, Maryia; Shishova, Maria; Martinec, Jan; Smolikova, Galina; Sharova, Elena; Demidchik, Vadim.

В: Functional Plant Biology, Том 46, № 6, 2019, стр. 533-542.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes

AU - Medvedev, Sergei

AU - Voronina, Olga

AU - Tankelyun, Olga

AU - Bilova, Tatiana

AU - Suslov, Dmitry

AU - Bankin, Mikhail

AU - Mackievic, Viera

AU - Makavitskaya, Maryia

AU - Shishova, Maria

AU - Martinec, Jan

AU - Smolikova, Galina

AU - Sharova, Elena

AU - Demidchik, Vadim

PY - 2019

Y1 - 2019

N2 - Phosphatidic acids (PAs) are a key intermediate in phospholipid biosynthesis, and a central element in numerous signalling pathways. Functions of PAs are related to their fundamental role in molecular interactions within cell membranes modifying membrane bending, budding, fission and fusion. Here we tested the hypothesis that PAs are capable of direct transport of ions across bio-membranes. We have demonstrated that PAs added to the maize plasma membrane vesicles induced ionophore-like transmembrane transport of Ca 2+ , H + and Mg 2+ . PA-induced Ca 2+ fluxes increased with an increasing PAs acyl chain unsaturation. For all the PAs analysed, the effect on Ca 2+ permeability increased with increasing pH (pH 8.0 > pH 7.2 > pH 6.0). The PA-induced Ca 2+ , Mg 2+ and H + permeability was also more pronounced in the endomembrane vesicles as compared with the plasma membrane vesicles. Addition of PA to protoplasts from Arabidopsis thaliana (L.) Heynh. roots constitutively expressing aequorin triggered elevation of the cytosolic Ca 2+ activity, indicating that the observed PA-dependent Ca 2+ transport occurs in intact plants.

AB - Phosphatidic acids (PAs) are a key intermediate in phospholipid biosynthesis, and a central element in numerous signalling pathways. Functions of PAs are related to their fundamental role in molecular interactions within cell membranes modifying membrane bending, budding, fission and fusion. Here we tested the hypothesis that PAs are capable of direct transport of ions across bio-membranes. We have demonstrated that PAs added to the maize plasma membrane vesicles induced ionophore-like transmembrane transport of Ca 2+ , H + and Mg 2+ . PA-induced Ca 2+ fluxes increased with an increasing PAs acyl chain unsaturation. For all the PAs analysed, the effect on Ca 2+ permeability increased with increasing pH (pH 8.0 > pH 7.2 > pH 6.0). The PA-induced Ca 2+ , Mg 2+ and H + permeability was also more pronounced in the endomembrane vesicles as compared with the plasma membrane vesicles. Addition of PA to protoplasts from Arabidopsis thaliana (L.) Heynh. roots constitutively expressing aequorin triggered elevation of the cytosolic Ca 2+ activity, indicating that the observed PA-dependent Ca 2+ transport occurs in intact plants.

KW - Ca signaling

KW - Ca transport

KW - H transport

KW - membrane vesicles

KW - phosphatidic acids

KW - protoplasts

KW - Ca 2+ transport

KW - H + transport

KW - Ca 2+ signaling

KW - Ca2+ signaling

KW - PHOSPHOLIPASE-D

KW - CHLORTETRACYCLINE

KW - ION

KW - MECHANISMS

KW - PLASMA-MEMBRANE

KW - MAGNESIUM

KW - DIVALENT-CATIONS

KW - CALCIUM

KW - STRESS

KW - H+ transport

KW - Ca2+ transport

KW - NONSELECTIVE CATION CHANNELS

UR - http://www.scopus.com/inward/record.url?scp=85062329766&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/phosphatidic-acids-mediate-transport-ca-2-h-through-plant-cell-membranes

U2 - 10.1071/FP18242

DO - 10.1071/FP18242

M3 - Article

AN - SCOPUS:85062329766

VL - 46

SP - 533

EP - 542

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 6

ER -