A multiproxy study of a new Pleistocene locality at Ivantzevo, Moscow Region, was conducted to reconstruct paleoenvironments from the Middle Pleistocene to the Last Pleniglacial. Lacustrine deposits and peat accumulated in a wetland within a fluvioglacial depression formed during the Dnieper–Moscow glaciation. Silts and clays were deposited during MIS 7 and the Moscow (Saale) Glaciation (MIS 6), while peat accumulation began in the Mikulino (Eemian) (MIS 5e). The wetland persisted for approximately fifty millennia, until the Middle Valdai (Weichselian). Interglacial peat deposits contain well-preserved pollen and macrofossils, and the recovered fossil insect assemblage is unique for European Russia. Chronology was established using multiple OSL and 230Th/U dates, combined with pollen-based correlations to type sections north and west of the region. The reconstructed ecosystem dynamics are divided into eleven stages. The transition from the last interglacial to the second stadial of the Valdai involved seven phases: (1) expansion of boreal spruce forest, (2) spread of thermophilic broad-leaved forests with hazel, (3) development of open forest–steppe ecosystems with groves of deciduous trees, (4) re-establishment of forest cover with birch and, later, mixed pine, spruce, and birch forests, (5) emergence of cold steppe combined with shrub-dominated tundra, (6) return of boreal spruce forest, and (7) abrupt replacement of forest by cold steppe and shrub tundra. Climatic reconstructions indicate that these ecosystem dynamics closely corresponded to changes in precipitation and aridity.