Overproduction of Sch9 leads to its aggregation and cell elongation in Saccharomyces cerevisiae

Polina Drozdova, Polina Lipaeva, Татьяна Рогоза, Galina Zhouravleva, Stanislav Bondarev

Результат исследований: Научные публикации в периодических изданияхстатья

Аннотация

The Sch9 kinase of Saccharomyces cerevisiae is one of the major TOR pathway effectors and regulates diverse processes in the cell. Sch9 belongs to the AGC kinase family. In human, amplification of AGC kinase genes is connected with cancer. However, not much is known about the effects of Sch9 overproduction in yeast cells. To fill this gap, we developed a model system to monitor subcellular location and aggregation state of overproduced Sch9 or its regions fused to a fluorescent protein. With this system, we showed that Sch9-YFP forms detergent-resistant aggregates, and multiple protein regions are responsible for this. This finding corroborated the fact that Sch9-YFP is visualized as various fluorescent foci. In addition, we found that Sch9 overproduction caused cell elongation, and this effect was determined by its C-terminal region containing kinase domains. The constructs we present can be exploited to create superior yeast-based model systems to study processes behind kinase overproduction in cancers.

Язык оригиналаанглийский
Номер статьиe0193726
Страницы (с-по)e0193726
Число страниц16
ЖурналPLoS ONE
Том13
Номер выпуска3
DOI
СостояниеОпубликовано - 1 мар 2018

Предметные области Scopus

  • Земледелие и биологические науки (все)
  • Биохимия, генетика и молекулярная биология (все)

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