TY - JOUR
T1 - Molecular phylogenetic analyses reveal a deep dichotomy in the conifer-inhabiting genusTrisetacus(Eriophyoidea
T2 - Nalepellidae), with the two lineages differing in their female genital morphology and host associations
AU - Chetverikov, Philipp E.
AU - Cvrkovic, Tatjana
AU - Efimov, Petr G.
AU - Klimov, Pavel B.
AU - Petanovic, Radmila U.
AU - Romanovich, Anna E.
AU - Schubert, Maria A.
AU - Sukhareva, Sogdiana
AU - Zukoff, Sarah N.
AU - Amrine, James
N1 - Funding Information:
Field work, comparative microscopy, and PCR were supported by Russian Foundation for Basic Research (RFBR grant # 19-04-00127 А) and research project of ZIN RAS (АААА-А19-119020790133-6). CLSM study and sequencing were carried out using the equipment of the ‘Development of Molecular and Cellular Technologies’ Resource Center and ‘Center for Microscopy and Microanalysis’ at St. Petersburg State University and were partially supported by Russian Science Foundation (Project 16-16-10011) and St. Petersburg State University (Project INI_2018-3 # 49711063).
Publisher Copyright:
© 2020, Springer Nature Switzerland AG.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - We analyzed the phylogenetic relationships of the genusTrisetacususing two genes [cytochromecoxidase subunit I (COI) and D1-D2 region of 28S rDNA (D1-D2 28S)], a representive taxon sampling (nearly 40% of known diversity), and a large set of close and distant outgroups. Our analyses suggest the presence of a dichotomy betweenTrisetacusassociated with Cupressaceae and Pinaceae. The following smaller molecular clades were found:Pin-1(bud mites, twig sheath mites, bark gall mites, and endoparasitic mites from pinaceans),Pin-2(needle sheath mites from pines),Pin-2a(putative Nearctic group of needle sheath mites),Pin-2b(putative Palearctic group of needle sheath mites),Cup-1and2(bud, cone, seed mites and mites living under bark scales from cupressaceans). The monophyly of the recently proposed subgenusBrevithecusnested within cladeCup-2was confirmed. Ancestral character reconstruction analyses recovered: (1) Pinaceae as the ancestral hosts of Nalepellidae andTrisetacus, (2) repetitive reductions of the spermathecal tube independently occurred in two lineages ofTrisetacusfrom Cupressaceae, and (3) several mite habitats on host (galls, cones, twig sheaths, seeds, inside leaves, and under scales) are evolutionarily derived states, whereas living in buds or needle sheaths are ancestral states forTrisetacuscladesCupandPin. Using confocal microscopy, we identified six basic types of the female internal genitalia ofTrisetacusbased on shapes of the spermatheca and spermathecal tube. These genitalic types are strongly correlated with lineages recovered by molecular phylogenetic analyses, suggesting that the female genital morphology is both evolutionarily conserved and is a factor influencing macroevolutionary patterns in this group of mites.
AB - We analyzed the phylogenetic relationships of the genusTrisetacususing two genes [cytochromecoxidase subunit I (COI) and D1-D2 region of 28S rDNA (D1-D2 28S)], a representive taxon sampling (nearly 40% of known diversity), and a large set of close and distant outgroups. Our analyses suggest the presence of a dichotomy betweenTrisetacusassociated with Cupressaceae and Pinaceae. The following smaller molecular clades were found:Pin-1(bud mites, twig sheath mites, bark gall mites, and endoparasitic mites from pinaceans),Pin-2(needle sheath mites from pines),Pin-2a(putative Nearctic group of needle sheath mites),Pin-2b(putative Palearctic group of needle sheath mites),Cup-1and2(bud, cone, seed mites and mites living under bark scales from cupressaceans). The monophyly of the recently proposed subgenusBrevithecusnested within cladeCup-2was confirmed. Ancestral character reconstruction analyses recovered: (1) Pinaceae as the ancestral hosts of Nalepellidae andTrisetacus, (2) repetitive reductions of the spermathecal tube independently occurred in two lineages ofTrisetacusfrom Cupressaceae, and (3) several mite habitats on host (galls, cones, twig sheaths, seeds, inside leaves, and under scales) are evolutionarily derived states, whereas living in buds or needle sheaths are ancestral states forTrisetacuscladesCupandPin. Using confocal microscopy, we identified six basic types of the female internal genitalia ofTrisetacusbased on shapes of the spermatheca and spermathecal tube. These genitalic types are strongly correlated with lineages recovered by molecular phylogenetic analyses, suggesting that the female genital morphology is both evolutionarily conserved and is a factor influencing macroevolutionary patterns in this group of mites.
KW - Gymnosperm pest
KW - Conifers
KW - Pinaceae
KW - Cupressaceae
KW - Confocal microscopy
KW - Phytoparasitic mites
KW - TRISETACUS SPECIES ERIOPHYOIDEA
KW - N. SP. ERIOPHYOIDEA
KW - INTERNAL GENITALIA
KW - CRYPTIC SPECIATION
KW - MITES ERIOPHYOIDEA
KW - ACARI
KW - PHYTOPTIDAE
KW - ACARIFORMES
KW - DIVERSITY
KW - POSITION
UR - http://www.scopus.com/inward/record.url?scp=85086164994&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/81a17382-629d-312b-a7d0-b4099b26a808/
U2 - 10.1007/s10493-020-00503-4
DO - 10.1007/s10493-020-00503-4
M3 - статья
VL - 81
SP - 287
EP - 316
JO - Experimental and Applied Acarology
JF - Experimental and Applied Acarology
SN - 0168-8162
IS - 3
ER -