Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI)

P. Zagata, M. Greczek-Stachura, S. Tarcz, M. Rautian

Результат исследований: Научные публикации в периодических изданияхстатьянаучная

1 цитирование (Scopus)

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Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen
Язык оригиналаанглийский
Страницы (с-по)1-7
ЖурналFOLIA BIOLOGICA-KRAKOW
Том63
Номер выпуска1
DOI
СостояниеОпубликовано - 2015

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    title = "Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI)",
    abstract = "Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen",
    keywords = "Paramecium bursaria, syngen, phylogeny",
    author = "P. Zagata and M. Greczek-Stachura and S. Tarcz and M. Rautian",
    year = "2015",
    doi = "10.3409/fb63_l.77",
    language = "English",
    volume = "63",
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    journal = "FOLIA BIOLOGICA-KRAKOW",
    issn = "0015-5497",
    publisher = "Institute of Systematics and Evolution of Animals",
    number = "1",

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    Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI). / Zagata, P.; Greczek-Stachura, M.; Tarcz, S.; Rautian, M.

    В: FOLIA BIOLOGICA-KRAKOW, Том 63, № 1, 2015, стр. 1-7.

    Результат исследований: Научные публикации в периодических изданияхстатьянаучная

    TY - JOUR

    T1 - Molecular Identification of Paramecium bursaria Syngens and Studies on Geographic Distribution using Mitochondrial Cytochrome C Oxidase Subunit I (COI)

    AU - Zagata, P.

    AU - Greczek-Stachura, M.

    AU - Tarcz, S.

    AU - Rautian, M.

    PY - 2015

    Y1 - 2015

    N2 - Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen

    AB - Paramecium bursaria is composed of five syngens that are morphologically indistinguishable but sexually isolated. The aim of the present study was to confirm by molecular methods (analyses of mitochondrial COI) the identification of P. bursaria syngens originating from different geographical locations. Phylograms constructed using both the neighbor-joining and maximum-likelihood methods based on a comparison of 34 sequences of P. bursaria strains and P. multimicronucleatum, P. caudatum and P.calkinsi strains used as outgroups revealed five clusters which correspond to results obtained previously by mating reaction. Our analysis shows the existence of 24 haplotypes for the COI gene sequence in the studied strains. The interspecies haplotype diversity was Hd = 0.967. We confirmed genetic differentiation between strains of P. bursaria and the occurrence of a correlation between geographical distribution and the correspondent syngen

    KW - Paramecium bursaria

    KW - syngen

    KW - phylogeny

    U2 - 10.3409/fb63_l.77

    DO - 10.3409/fb63_l.77

    M3 - Article

    VL - 63

    SP - 1

    EP - 7

    JO - FOLIA BIOLOGICA-KRAKOW

    JF - FOLIA BIOLOGICA-KRAKOW

    SN - 0015-5497

    IS - 1

    ER -