Mild osmotic stress in intertidal gastropods Littorina saxatilis and Littorina obtusata (Mollusca: Caenogastropoda): a proteomic analysis

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

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Salinity is a crucial abiotic environmental factor for marine animals, affecting their physiology and geographic ranges. Deviation of environmental salinity from the organismal optimum range results in an osmotic stress in osmoconformers, which keep their fluids isotonic to the environment. The ability to overcome such stress is critical for animals inhabiting areas with considerable salinity variation, such as intertidal areas. In this study, we compared the reaction to mild water freshening (from 24 to 14 ‰) in two related species of intertidal snails, Littorina saxatilis and L. obtusata, with respect to several aspects: survival, behavior and proteomic changes. Among these species, L. saxatilis is more tolerant to low salinity and survives in estuaries. We found out that the response of these species was much milder (with no mortality or isolation reaction observed) and involved weaker proteomic changes than during acute stress (freshening from 24 to 10 ‰), characterized earlier. The proteomic response of the second species, L. obtusata, was weaker (6 % vs 10 % of regulated proteins) than that of L. saxatilis and engaged a mostly different set of proteins. Among proteins potentially involved in adaptation to low salinity, we identified enzymes of energetic metabolism and antioxidant response, chaperones, proteins of extracellular matrix and cytoskeleton, ion channels and regulators of cell growth and proliferation.
Язык оригиналаанглийский
Номер статьи305
Страницы (с-по)202-213
Число страниц12
ЖурналBiological Communications
Том62
Номер выпуска3
DOI
СостояниеОпубликовано - 25 ноя 2017

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    @article{2f78feafcbce49329a2f119975ed375e,
    title = "Mild osmotic stress in intertidal gastropods Littorina saxatilis and Littorina obtusata (Mollusca: Caenogastropoda): a proteomic analysis",
    abstract = "Salinity is a crucial abiotic environmental factor for marine animals, affecting their physiology and geographic ranges. Deviation of environmental salinity from the organismal optimum range results in an osmotic stress in osmoconformers, which keep their fluids isotonic to the environment. The ability to overcome such stress is critical for animals inhabiting areas with considerable salinity variation, such as intertidal areas. In this study, we compared the reaction to mild water freshening (from 24 to 14 ‰) in two related species of intertidal snails, Littorina saxatilis and L. obtusata, with respect to several aspects: survival, behavior and proteomic changes. Among these species, L. saxatilis is more tolerant to low salinity and survives in estuaries. We found out that the response of these species was much milder (with no mortality or isolation reaction observed) and involved weaker proteomic changes than during acute stress (freshening from 24 to 10 ‰), characterized earlier. The proteomic response of the second species, L. obtusata, was weaker (6 {\%} vs 10 {\%} of regulated proteins) than that of L. saxatilis and engaged a mostly different set of proteins. Among proteins potentially involved in adaptation to low salinity, we identified enzymes of energetic metabolism and antioxidant response, chaperones, proteins of extracellular matrix and cytoskeleton, ion channels and regulators of cell growth and proliferation.",
    keywords = "salinity adaptation, osmotic stress, proteomic analysis, intertidal molluscs, periwinkles, Littorina, 2D-DIGE",
    author = "Muraeva, {Olga A.} and Мальцева, {Арина Леонидовна} and Варфоломеева, {Марина Александровна} and Михайлова, {Наталья Аркадьевна} and Гранович, {Андрей Игоревич}",
    year = "2017",
    month = "11",
    day = "25",
    doi = "10.21638/11701/spbu03.2017.305",
    language = "English",
    volume = "62",
    pages = "202--213",
    journal = "Biological Communications",
    issn = "2542-2154",
    publisher = "Издательство Санкт-Петербургского университета",
    number = "3",

    }

    Mild osmotic stress in intertidal gastropods Littorina saxatilis and Littorina obtusata (Mollusca: Caenogastropoda): a proteomic analysis. / Muraeva, Olga A.; Мальцева, Арина Леонидовна; Варфоломеева, Марина Александровна; Михайлова, Наталья Аркадьевна; Гранович, Андрей Игоревич.

    В: Biological Communications, Том 62, № 3, 305, 25.11.2017, стр. 202-213.

    Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

    TY - JOUR

    T1 - Mild osmotic stress in intertidal gastropods Littorina saxatilis and Littorina obtusata (Mollusca: Caenogastropoda): a proteomic analysis

    AU - Muraeva, Olga A.

    AU - Мальцева, Арина Леонидовна

    AU - Варфоломеева, Марина Александровна

    AU - Михайлова, Наталья Аркадьевна

    AU - Гранович, Андрей Игоревич

    PY - 2017/11/25

    Y1 - 2017/11/25

    N2 - Salinity is a crucial abiotic environmental factor for marine animals, affecting their physiology and geographic ranges. Deviation of environmental salinity from the organismal optimum range results in an osmotic stress in osmoconformers, which keep their fluids isotonic to the environment. The ability to overcome such stress is critical for animals inhabiting areas with considerable salinity variation, such as intertidal areas. In this study, we compared the reaction to mild water freshening (from 24 to 14 ‰) in two related species of intertidal snails, Littorina saxatilis and L. obtusata, with respect to several aspects: survival, behavior and proteomic changes. Among these species, L. saxatilis is more tolerant to low salinity and survives in estuaries. We found out that the response of these species was much milder (with no mortality or isolation reaction observed) and involved weaker proteomic changes than during acute stress (freshening from 24 to 10 ‰), characterized earlier. The proteomic response of the second species, L. obtusata, was weaker (6 % vs 10 % of regulated proteins) than that of L. saxatilis and engaged a mostly different set of proteins. Among proteins potentially involved in adaptation to low salinity, we identified enzymes of energetic metabolism and antioxidant response, chaperones, proteins of extracellular matrix and cytoskeleton, ion channels and regulators of cell growth and proliferation.

    AB - Salinity is a crucial abiotic environmental factor for marine animals, affecting their physiology and geographic ranges. Deviation of environmental salinity from the organismal optimum range results in an osmotic stress in osmoconformers, which keep their fluids isotonic to the environment. The ability to overcome such stress is critical for animals inhabiting areas with considerable salinity variation, such as intertidal areas. In this study, we compared the reaction to mild water freshening (from 24 to 14 ‰) in two related species of intertidal snails, Littorina saxatilis and L. obtusata, with respect to several aspects: survival, behavior and proteomic changes. Among these species, L. saxatilis is more tolerant to low salinity and survives in estuaries. We found out that the response of these species was much milder (with no mortality or isolation reaction observed) and involved weaker proteomic changes than during acute stress (freshening from 24 to 10 ‰), characterized earlier. The proteomic response of the second species, L. obtusata, was weaker (6 % vs 10 % of regulated proteins) than that of L. saxatilis and engaged a mostly different set of proteins. Among proteins potentially involved in adaptation to low salinity, we identified enzymes of energetic metabolism and antioxidant response, chaperones, proteins of extracellular matrix and cytoskeleton, ion channels and regulators of cell growth and proliferation.

    KW - salinity adaptation

    KW - osmotic stress

    KW - proteomic analysis

    KW - intertidal molluscs

    KW - periwinkles

    KW - Littorina

    KW - 2D-DIGE

    U2 - 10.21638/11701/spbu03.2017.305

    DO - 10.21638/11701/spbu03.2017.305

    M3 - Article

    VL - 62

    SP - 202

    EP - 213

    JO - Biological Communications

    JF - Biological Communications

    SN - 2542-2154

    IS - 3

    M1 - 305

    ER -