Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization

Natalia Osmolovskaya, Julia Shumilina, Ahyoung Kim, Anna Didio, Tatiana Grishina, Tatiana Bilova, Olga A. Keltsieva, Vladimir Zhukov, Igor Tikhonovich, Elena Tarakhovskaya, Andrej Frolov, Ludger A. Wessjohann

Результат исследований: Научные публикации в периодических изданияхОбзорная статья

6 Цитирования (Scopus)
2 Downloads (Pure)

Выдержка

Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecular, cellular, and organism levels. To understand the underlying mechanisms of drought tolerance, adequate stress models and arrays of reliable stress markers are required. Therefore, in this review we comprehensively address currently available models of drought stress, based on culturing plants in soil, hydroponically, or in agar culture, and critically discuss advantages and limitations of each design. We also address the methodology of drought stress characterization and discuss it in the context of real experimental approaches. Further, we highlight the trends of methodological developments in drought stress research, i.e., complementing conventional tests with quantification of phytohormones and reactive oxygen species (ROS), measuring antioxidant enzyme activities, and comprehensively profiling transcriptome, proteome, and metabolome.

Язык оригиналаАнглийский
Номер статьи4089
Число страниц25
ЖурналInternational Journal of Molecular Sciences
Том19
Номер выпуска12
Ранняя дата в режиме онлайн2018
DOI
СостояниеОпубликовано - 17 дек 2018

Предметные области Scopus

  • Биохимия
  • Молекулярная биология
  • Спектроскопия
  • Катализ
  • Неорганическая химия
  • Прикладные компьютерные науки
  • Физическая и теоретическая химия
  • Органическая химия

Ключевые слова

  • drought stress; drought models; drought tolerance; oxidative stress; phytohormones; polyethylene glycol (PEG); stress markers

Цитировать

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title = "Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization",
abstract = "Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecular, cellular, and organism levels. To understand the underlying mechanisms of drought tolerance, adequate stress models and arrays of reliable stress markers are required. Therefore, in this review we comprehensively address currently available models of drought stress, based on culturing plants in soil, hydroponically, or in agar culture, and critically discuss advantages and limitations of each design. We also address the methodology of drought stress characterization and discuss it in the context of real experimental approaches. Further, we highlight the trends of methodological developments in drought stress research, i.e., complementing conventional tests with quantification of phytohormones and reactive oxygen species (ROS), measuring antioxidant enzyme activities, and comprehensively profiling transcriptome, proteome, and metabolome.",
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author = "Natalia Osmolovskaya and Julia Shumilina and Ahyoung Kim and Anna Didio and Tatiana Grishina and Tatiana Bilova and Keltsieva, {Olga A.} and Vladimir Zhukov and Igor Tikhonovich and Elena Tarakhovskaya and Andrej Frolov and Wessjohann, {Ludger A.}",
year = "2018",
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Methodology of Drought Stress Research : Experimental Setup and Physiological Characterization. / Osmolovskaya, Natalia; Shumilina, Julia; Kim, Ahyoung; Didio, Anna; Grishina, Tatiana; Bilova, Tatiana; Keltsieva, Olga A.; Zhukov, Vladimir; Tikhonovich, Igor; Tarakhovskaya, Elena; Frolov, Andrej; Wessjohann, Ludger A.

В: International Journal of Molecular Sciences, Том 19, № 12, 4089, 17.12.2018.

Результат исследований: Научные публикации в периодических изданияхОбзорная статья

TY - JOUR

T1 - Methodology of Drought Stress Research

T2 - Experimental Setup and Physiological Characterization

AU - Osmolovskaya, Natalia

AU - Shumilina, Julia

AU - Kim, Ahyoung

AU - Didio, Anna

AU - Grishina, Tatiana

AU - Bilova, Tatiana

AU - Keltsieva, Olga A.

AU - Zhukov, Vladimir

AU - Tikhonovich, Igor

AU - Tarakhovskaya, Elena

AU - Frolov, Andrej

AU - Wessjohann, Ludger A.

PY - 2018/12/17

Y1 - 2018/12/17

N2 - Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecular, cellular, and organism levels. To understand the underlying mechanisms of drought tolerance, adequate stress models and arrays of reliable stress markers are required. Therefore, in this review we comprehensively address currently available models of drought stress, based on culturing plants in soil, hydroponically, or in agar culture, and critically discuss advantages and limitations of each design. We also address the methodology of drought stress characterization and discuss it in the context of real experimental approaches. Further, we highlight the trends of methodological developments in drought stress research, i.e., complementing conventional tests with quantification of phytohormones and reactive oxygen species (ROS), measuring antioxidant enzyme activities, and comprehensively profiling transcriptome, proteome, and metabolome.

AB - Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecular, cellular, and organism levels. To understand the underlying mechanisms of drought tolerance, adequate stress models and arrays of reliable stress markers are required. Therefore, in this review we comprehensively address currently available models of drought stress, based on culturing plants in soil, hydroponically, or in agar culture, and critically discuss advantages and limitations of each design. We also address the methodology of drought stress characterization and discuss it in the context of real experimental approaches. Further, we highlight the trends of methodological developments in drought stress research, i.e., complementing conventional tests with quantification of phytohormones and reactive oxygen species (ROS), measuring antioxidant enzyme activities, and comprehensively profiling transcriptome, proteome, and metabolome.

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KW - HEAT-SHOCK PROTEINS

KW - ABIOTIC STRESS

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KW - OSMOTIC-STRESS

KW - GENE-EXPRESSION

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KW - CHLOROPHYLL FLUORESCENCE

KW - POLYETHYLENE GLYCOL-6000

KW - OXIDATIVE-DEGRADATION

KW - Oxidative stress

KW - Drought models

KW - Stress markers

KW - Drought tolerance

KW - Polyethylene glycol (PEG)

KW - Drought stress

KW - Phytohormones

KW - drought stress; drought models; drought tolerance; oxidative stress; phytohormones; polyethylene glycol (PEG); stress markers

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DO - 10.3390/ijms19124089

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

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