Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

4 Цитирования (Scopus)

Выдержка

During mixotrophic batch culturing, the microalga Chlamydomonas reinhardtii goes through S-shaped growth kinetics and cells consistently transit from division to death. Photosynthesis and respiration had two simultaneous maxima at the first half of the exponential growth phase and at the border of the stationary phase. GC-MS analysis detected about 300 compounds of which about 100 were identified. Differences in metabolite profiles were observed between cells sampled at different time points of the growth curve. Several data mining methods clearly indicated that metabolomes tended to group together in a time-dependent manner. Clustering analysis revealed three major groups of metabolites varied in concentration dynamic. The alterations in lipophilic compounds and carbohydrates took place even within the same phase of culture growth, which indicated complex rearrangements in cell biochemistry and physiology. Special attention was paid to alterations in the ratios between metabolite groups. The most significant ratio changes were discovered for pools of amino and fatty acids.
Язык оригиналаанглийский
Страницы (с-по)803-818
Число страниц16
ЖурналJournal of Applied Phycology
Том30
Номер выпуска2
DOI
СостояниеОпубликовано - апр 2018

Предметные области Scopus

  • Биохимия, генетика и молекулярная биология (все)
  • Акванаука
  • Прикладная ботаника

Цитировать

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title = "Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development",
abstract = "During mixotrophic batch culturing, the microalga Chlamydomonas reinhardtii goes through S-shaped growth kinetics and cells consistently transit from division to death. Photosynthesis and respiration had two simultaneous maxima at the first half of the exponential growth phase and at the border of the stationary phase. GC-MS analysis detected about 300 compounds of which about 100 were identified. Differences in metabolite profiles were observed between cells sampled at different time points of the growth curve. Several data mining methods clearly indicated that metabolomes tended to group together in a time-dependent manner. Clustering analysis revealed three major groups of metabolites varied in concentration dynamic. The alterations in lipophilic compounds and carbohydrates took place even within the same phase of culture growth, which indicated complex rearrangements in cell biochemistry and physiology. Special attention was paid to alterations in the ratios between metabolite groups. The most significant ratio changes were discovered for pools of amino and fatty acids.",
keywords = "Batch culture, Chlamydomonas reinhardtii, Metabolomics, Multivariate statistics, Photosynthesis, Respiration",
author = "Roman Puzanskiy and Elena Tarakhovskaya and Alexey Shavarda and Maria Shishova",
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month = "4",
doi = "10.1007/s10811-017-1326-9",
language = "English",
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Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development. / Puzanskiy, Roman; Tarakhovskaya, Elena; Shavarda, Alexey; Shishova, Maria.

В: Journal of Applied Phycology, Том 30, № 2, 04.2018, стр. 803-818.

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

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T1 - Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development

AU - Puzanskiy, Roman

AU - Tarakhovskaya, Elena

AU - Shavarda, Alexey

AU - Shishova, Maria

PY - 2018/4

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N2 - During mixotrophic batch culturing, the microalga Chlamydomonas reinhardtii goes through S-shaped growth kinetics and cells consistently transit from division to death. Photosynthesis and respiration had two simultaneous maxima at the first half of the exponential growth phase and at the border of the stationary phase. GC-MS analysis detected about 300 compounds of which about 100 were identified. Differences in metabolite profiles were observed between cells sampled at different time points of the growth curve. Several data mining methods clearly indicated that metabolomes tended to group together in a time-dependent manner. Clustering analysis revealed three major groups of metabolites varied in concentration dynamic. The alterations in lipophilic compounds and carbohydrates took place even within the same phase of culture growth, which indicated complex rearrangements in cell biochemistry and physiology. Special attention was paid to alterations in the ratios between metabolite groups. The most significant ratio changes were discovered for pools of amino and fatty acids.

AB - During mixotrophic batch culturing, the microalga Chlamydomonas reinhardtii goes through S-shaped growth kinetics and cells consistently transit from division to death. Photosynthesis and respiration had two simultaneous maxima at the first half of the exponential growth phase and at the border of the stationary phase. GC-MS analysis detected about 300 compounds of which about 100 were identified. Differences in metabolite profiles were observed between cells sampled at different time points of the growth curve. Several data mining methods clearly indicated that metabolomes tended to group together in a time-dependent manner. Clustering analysis revealed three major groups of metabolites varied in concentration dynamic. The alterations in lipophilic compounds and carbohydrates took place even within the same phase of culture growth, which indicated complex rearrangements in cell biochemistry and physiology. Special attention was paid to alterations in the ratios between metabolite groups. The most significant ratio changes were discovered for pools of amino and fatty acids.

KW - Batch culture

KW - Chlamydomonas reinhardtii

KW - Metabolomics

KW - Multivariate statistics

KW - Photosynthesis

KW - Respiration

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