Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules

Anna Edlund, Neha Garg, Hosein Mohimani, Alexey Gurevich, Xuesong He, Wenyuan Shi, Pieter C. Dorrestein, Jeffrey S. McLean

Результат исследований: Научные публикации в периодических изданияхстатья

8 Цитирования (Scopus)

Выдержка

Recent research indicates that the human microbiota play key roles in maintaining health by providing essential nutrients, providing immune education, and preventing pathogen expansion. Processes underlying the transition from a healthy human microbiome to a disease-associated microbiome are poorly understood, partially because of the potential influences from a wide diversity of bacterium-derived compounds that are illy defined. Here, we present the analysis of peptidic small molecules (SMs) secreted from bacteria and viewed from a temporal perspective. Through comparative analysis of mass spectral profiles from a collection of cultured oral isolates and an established in vitro multispecies oral community, we found that the production of SMs both delineates a temporal expression pattern and allows discrimination between bacterial isolates at the species level. Importantly, the majority of the identified molecules were of unknown identity, and only ∼2.2% could be annotated and classified. The catalogue of bacterially produced SMs we obtained in this study reveals an undiscovered molecular world for which compound isolation and ecosystem testing will facilitate a better understanding of their roles in human health and disease.

Язык оригиналаанглийский
Номер статьиe00058
ЖурналmSystems
Том2
Номер выпуска4
DOI
СостояниеОпубликовано - 29 авг 2017

Отпечаток

Microbiota
Dermatoglyphics
Fingerprint
mouth
Molecules
health and disease
bacterium
Bacteria
Health
pathogen
bacteria
education
human diseases
Ecosystem
human health
nutrient
ecosystem
Pathogens
Nutrients
Comparative Analysis

Предметные области Scopus

  • Микробиология
  • Физиология
  • Биохимия
  • Экология, эволюция поведение и систематика
  • Моделирование и симуляция
  • Молекулярная биология
  • Генетика
  • Прикладные компьютерные науки

Цитировать

Edlund, A., Garg, N., Mohimani, H., Gurevich, A., He, X., Shi, W., ... McLean, J. S. (2017). Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules. mSystems, 2(4), [e00058]. https://doi.org/10.1128/mSystems.00058-17
Edlund, Anna ; Garg, Neha ; Mohimani, Hosein ; Gurevich, Alexey ; He, Xuesong ; Shi, Wenyuan ; Dorrestein, Pieter C. ; McLean, Jeffrey S. / Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules. В: mSystems. 2017 ; Том 2, № 4.
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title = "Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules",
abstract = "Recent research indicates that the human microbiota play key roles in maintaining health by providing essential nutrients, providing immune education, and preventing pathogen expansion. Processes underlying the transition from a healthy human microbiome to a disease-associated microbiome are poorly understood, partially because of the potential influences from a wide diversity of bacterium-derived compounds that are illy defined. Here, we present the analysis of peptidic small molecules (SMs) secreted from bacteria and viewed from a temporal perspective. Through comparative analysis of mass spectral profiles from a collection of cultured oral isolates and an established in vitro multispecies oral community, we found that the production of SMs both delineates a temporal expression pattern and allows discrimination between bacterial isolates at the species level. Importantly, the majority of the identified molecules were of unknown identity, and only ∼2.2{\%} could be annotated and classified. The catalogue of bacterially produced SMs we obtained in this study reveals an undiscovered molecular world for which compound isolation and ecosystem testing will facilitate a better understanding of their roles in human health and disease.",
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Edlund, A, Garg, N, Mohimani, H, Gurevich, A, He, X, Shi, W, Dorrestein, PC & McLean, JS 2017, 'Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules', mSystems, том. 2, № 4, e00058. https://doi.org/10.1128/mSystems.00058-17

Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules. / Edlund, Anna; Garg, Neha; Mohimani, Hosein; Gurevich, Alexey; He, Xuesong; Shi, Wenyuan; Dorrestein, Pieter C.; McLean, Jeffrey S.

В: mSystems, Том 2, № 4, e00058, 29.08.2017.

Результат исследований: Научные публикации в периодических изданияхстатья

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T1 - Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules

AU - Edlund, Anna

AU - Garg, Neha

AU - Mohimani, Hosein

AU - Gurevich, Alexey

AU - He, Xuesong

AU - Shi, Wenyuan

AU - Dorrestein, Pieter C.

AU - McLean, Jeffrey S.

PY - 2017/8/29

Y1 - 2017/8/29

N2 - Recent research indicates that the human microbiota play key roles in maintaining health by providing essential nutrients, providing immune education, and preventing pathogen expansion. Processes underlying the transition from a healthy human microbiome to a disease-associated microbiome are poorly understood, partially because of the potential influences from a wide diversity of bacterium-derived compounds that are illy defined. Here, we present the analysis of peptidic small molecules (SMs) secreted from bacteria and viewed from a temporal perspective. Through comparative analysis of mass spectral profiles from a collection of cultured oral isolates and an established in vitro multispecies oral community, we found that the production of SMs both delineates a temporal expression pattern and allows discrimination between bacterial isolates at the species level. Importantly, the majority of the identified molecules were of unknown identity, and only ∼2.2% could be annotated and classified. The catalogue of bacterially produced SMs we obtained in this study reveals an undiscovered molecular world for which compound isolation and ecosystem testing will facilitate a better understanding of their roles in human health and disease.

AB - Recent research indicates that the human microbiota play key roles in maintaining health by providing essential nutrients, providing immune education, and preventing pathogen expansion. Processes underlying the transition from a healthy human microbiome to a disease-associated microbiome are poorly understood, partially because of the potential influences from a wide diversity of bacterium-derived compounds that are illy defined. Here, we present the analysis of peptidic small molecules (SMs) secreted from bacteria and viewed from a temporal perspective. Through comparative analysis of mass spectral profiles from a collection of cultured oral isolates and an established in vitro multispecies oral community, we found that the production of SMs both delineates a temporal expression pattern and allows discrimination between bacterial isolates at the species level. Importantly, the majority of the identified molecules were of unknown identity, and only ∼2.2% could be annotated and classified. The catalogue of bacterially produced SMs we obtained in this study reveals an undiscovered molecular world for which compound isolation and ecosystem testing will facilitate a better understanding of their roles in human health and disease.

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KW - Lactobacillus

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KW - Peptidic small molecules

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