Metabolic alterations in male-sterile potato as compared to male-fertile

Maria Shishova, Roman Puzanskiy, Olga Gavrilova, Shamuhommed Kurbanniazov, Kirill Demchenko, Vladislav Yemelyanov, Galina Pendinen, Alexey Shavarda, Tatjana Gavrilenko

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

Выдержка

The common potato, Solanum tuberosum L., is the fourth most important agricultural crop worldwide. Until recently, vegetative propagation by tubers has been the main method of potato cultivation. A shift of interest to sexual potato reproduction by true botanical seeds is due to the appearance of a new hybrid seed breeding strategy whose successful application for many crop species has been supported by male sterility. This investigation was focused on the study of differences in the metabolite profiles of anthers at the mature pollen stage from male-fertile and male-sterile genotypes of S. tuberosum. Application of gas chromatography coupled with a mass spectrometry method allowed detection of metabolic profiles for 192 compounds. Further data analysis with several libraries fully identified 75 metabolites; a similar amount was defined up to the classes. Metabolic profiles in the anthers of fertile genotypes were significantly distinguished from male-sterile ones by the accumulation of carbohydrates, while the anthers of sterile genotypes contained a higher amount of amino acids. In comparison with male-fertile plants, male-sterile genotypes had undeveloped pollen grain characters; i.e., smaller grain size, a thicker exine, “permanent tetrads” that failed to disintegrate into microspores, and the absence of pollen apertures that might be due to a disorder in the metabolism of carbohydrates and fatty acids.

Язык оригиналаанглийский
Номер статьи24
ЖурналMetabolites
Том9
Номер выпуска2
DOI
СостояниеОпубликовано - 2019

Отпечаток

Solanum tuberosum
Metabolites
Crops
Seed
Carbohydrates
Pollen
Genotype
Metabolism
Gas chromatography
Metabolome
Mass spectrometry
Fatty Acids
Seeds
Amino Acids
Agricultural Crops
Male Infertility
Carbohydrate Metabolism
Gas Chromatography
Libraries
Breeding

Предметные области Scopus

  • Эндокринология, диабет и метаболизм
  • Биохимия
  • Молекулярная биология

Цитировать

Shishova, Maria ; Puzanskiy, Roman ; Gavrilova, Olga ; Kurbanniazov, Shamuhommed ; Demchenko, Kirill ; Yemelyanov, Vladislav ; Pendinen, Galina ; Shavarda, Alexey ; Gavrilenko, Tatjana. / Metabolic alterations in male-sterile potato as compared to male-fertile. В: Metabolites. 2019 ; Том 9, № 2.
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title = "Metabolic alterations in male-sterile potato as compared to male-fertile",
abstract = "The common potato, Solanum tuberosum L., is the fourth most important agricultural crop worldwide. Until recently, vegetative propagation by tubers has been the main method of potato cultivation. A shift of interest to sexual potato reproduction by true botanical seeds is due to the appearance of a new hybrid seed breeding strategy whose successful application for many crop species has been supported by male sterility. This investigation was focused on the study of differences in the metabolite profiles of anthers at the mature pollen stage from male-fertile and male-sterile genotypes of S. tuberosum. Application of gas chromatography coupled with a mass spectrometry method allowed detection of metabolic profiles for 192 compounds. Further data analysis with several libraries fully identified 75 metabolites; a similar amount was defined up to the classes. Metabolic profiles in the anthers of fertile genotypes were significantly distinguished from male-sterile ones by the accumulation of carbohydrates, while the anthers of sterile genotypes contained a higher amount of amino acids. In comparison with male-fertile plants, male-sterile genotypes had undeveloped pollen grain characters; i.e., smaller grain size, a thicker exine, “permanent tetrads” that failed to disintegrate into microspores, and the absence of pollen apertures that might be due to a disorder in the metabolism of carbohydrates and fatty acids.",
keywords = "Male sterility, Metabolite profiles, Potato, Solanum tuberosum, Untargeted metabolomics",
author = "Maria Shishova and Roman Puzanskiy and Olga Gavrilova and Shamuhommed Kurbanniazov and Kirill Demchenko and Vladislav Yemelyanov and Galina Pendinen and Alexey Shavarda and Tatjana Gavrilenko",
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Metabolic alterations in male-sterile potato as compared to male-fertile. / Shishova, Maria; Puzanskiy, Roman; Gavrilova, Olga; Kurbanniazov, Shamuhommed; Demchenko, Kirill; Yemelyanov, Vladislav; Pendinen, Galina; Shavarda, Alexey; Gavrilenko, Tatjana.

В: Metabolites, Том 9, № 2, 24, 2019.

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

TY - JOUR

T1 - Metabolic alterations in male-sterile potato as compared to male-fertile

AU - Shishova, Maria

AU - Puzanskiy, Roman

AU - Gavrilova, Olga

AU - Kurbanniazov, Shamuhommed

AU - Demchenko, Kirill

AU - Yemelyanov, Vladislav

AU - Pendinen, Galina

AU - Shavarda, Alexey

AU - Gavrilenko, Tatjana

PY - 2019

Y1 - 2019

N2 - The common potato, Solanum tuberosum L., is the fourth most important agricultural crop worldwide. Until recently, vegetative propagation by tubers has been the main method of potato cultivation. A shift of interest to sexual potato reproduction by true botanical seeds is due to the appearance of a new hybrid seed breeding strategy whose successful application for many crop species has been supported by male sterility. This investigation was focused on the study of differences in the metabolite profiles of anthers at the mature pollen stage from male-fertile and male-sterile genotypes of S. tuberosum. Application of gas chromatography coupled with a mass spectrometry method allowed detection of metabolic profiles for 192 compounds. Further data analysis with several libraries fully identified 75 metabolites; a similar amount was defined up to the classes. Metabolic profiles in the anthers of fertile genotypes were significantly distinguished from male-sterile ones by the accumulation of carbohydrates, while the anthers of sterile genotypes contained a higher amount of amino acids. In comparison with male-fertile plants, male-sterile genotypes had undeveloped pollen grain characters; i.e., smaller grain size, a thicker exine, “permanent tetrads” that failed to disintegrate into microspores, and the absence of pollen apertures that might be due to a disorder in the metabolism of carbohydrates and fatty acids.

AB - The common potato, Solanum tuberosum L., is the fourth most important agricultural crop worldwide. Until recently, vegetative propagation by tubers has been the main method of potato cultivation. A shift of interest to sexual potato reproduction by true botanical seeds is due to the appearance of a new hybrid seed breeding strategy whose successful application for many crop species has been supported by male sterility. This investigation was focused on the study of differences in the metabolite profiles of anthers at the mature pollen stage from male-fertile and male-sterile genotypes of S. tuberosum. Application of gas chromatography coupled with a mass spectrometry method allowed detection of metabolic profiles for 192 compounds. Further data analysis with several libraries fully identified 75 metabolites; a similar amount was defined up to the classes. Metabolic profiles in the anthers of fertile genotypes were significantly distinguished from male-sterile ones by the accumulation of carbohydrates, while the anthers of sterile genotypes contained a higher amount of amino acids. In comparison with male-fertile plants, male-sterile genotypes had undeveloped pollen grain characters; i.e., smaller grain size, a thicker exine, “permanent tetrads” that failed to disintegrate into microspores, and the absence of pollen apertures that might be due to a disorder in the metabolism of carbohydrates and fatty acids.

KW - Male sterility

KW - Metabolite profiles

KW - Potato

KW - Solanum tuberosum

KW - Untargeted metabolomics

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JO - Metabolites

JF - Metabolites

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