Macroporous polymer monoliths for affinity chromatography and solid-phase enzyme processing

Результат исследований: Публикации в книгах, отчётах, сборниках, трудах конференцийглава/раздел

Аннотация

Nowadays, monolithic stationary phases, because of their special morphology and enormous permeability, are widely used for the development and realization of fast dynamic and static processes based on the mass transition between liquid and solid phases. These are liquid chromatography, solid-phase synthesis, microarrays, flow-through enzyme reactors, etc. High-performance liquid chromatography on monoliths, including the bioaffinity mode, represents unique technique appropriate for fast and efficient separation of biological (macro)molecules of different sizes and shapes (proteins, nucleic acids, peptides), as well as such supramolecular systems as viruses. In the edited chapter, the examples of the application of commercially available macroporous monoliths for modern affinity processing are presented. In particular, the original methods developed for efficient isolation and fractionation of monospecific antibodies from rabbit blood sera, the possibility of simultaneous affinity separation of protein G and serum albumin from human serum, the isolation of recombinant products, such as protein G and tissue plasminogen activator, respectively, are described in detail. The suggested and realized multifunctional fractionation of polyclonal pools of antibodies by the combination of several short monolithic columns (disks) with different affinity functionalities stacked in the same cartridge represents the original and practically valuable method that can be used in biotechnology. In addition, macroporous monoliths were adapted to the immobilization of such different enzymes as polynucleotide phosphorylase, ribonuclease A, α-chymotrypsin, chitinolytic biocatalysts, β-xylosidase, and β-xylanase. The possibility of use of immobilized enzyme reactors based on monoliths for different purposes is demonstrated.

Язык оригиналаанглийский
Название основной публикацииProtein Downstream Processing
Подзаголовок основной публикацииDesign, Development, and Application of High and Low-Resolution Methods
РедакторыNikolaos E. Labrou
ИздательHumana Press
Страницы251-284
ISBN (электронное издание)978-1-0716-0775-6
ISBN (печатное издание)978-1-0716-0774-9
DOI
СостояниеОпубликовано - 2021

Серия публикаций

НазваниеMethods in Molecular Biology
Том2178
ISSN (печатное издание)1064-3745
ISSN (электронное издание)1940-6029

Предметные области Scopus

  • Молекулярная биология
  • Генетика

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