M60-like metalloprotease domain of the Escherichia coli YghJ protein forms amyloid fibrils

Mikhail V Belousov, Stanislav A Bondarev, Anastasiia O Kosolapova, Kirill S Antonets, Anna I Sulatskaya, Maksim I Sulatsky, Galina A Zhouravleva, Irina M Kuznetsova, Konstantin K Turoverov, Anton A Nizhnikov

Результат исследований: Научные публикации в периодических изданияхстатья

5 Цитирования (Scopus)

Аннотация

Amyloids are protein fibrils with a characteristic spatial structure. Amyloids were long perceived as the pathogens involved in a set of lethal diseases in humans and animals. In recent decades, it has become clear that amyloids represent a quaternary protein structure that is not only pathological but also functionally important and is widely used by different organisms, ranging from archaea to animals, to implement diverse biological functions. The greatest biological variety of amyloids is found in prokaryotes, where they control the formation of biofilms and cell wall sheaths, facilitate the overcoming of surface tension, and regulate the metabolism of toxins. Several amyloid proteins were identified in the important model, biotechnological and pathogenic bacterium Escherichia coli. In previous studies, using a method for the proteomic screening and identification of amyloids, we identified 61 potentially amyloidogenic proteins in the proteome of E. coli. Among these proteins, YghJ was the most enriched with bioinformatically predicted amyloidogenic regions. YghJ is a lipoprotein with a zinc metalloprotease M60-like domain that is involved in mucin degradation in the intestine as well as in proinflammatory responses. In this study, we analyzed the amyloid properties of the YghJ M60-like domain and demonstrated that it forms amyloid-like fibrils in vitro and in vivo.

Язык оригиналаанглийский
Номер статьиe0191317
Страницы (с-по)e0191317
Число страниц17
ЖурналPLoS ONE
Том13
Номер выпуска1
DOI
СостояниеОпубликовано - 2018

Предметные области Scopus

  • Земледелие и биологические науки (все)
  • Биохимия, генетика и молекулярная биология (все)

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