Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles

Oleg Gorshkov, Tatyana Chernova, Natalia Mokshina, Natalia Gogoleva, Dmitry Suslov, Alexander Tkachenko, Tatyana Gorshkova

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, andmiR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.

Язык оригиналаанглийский
Номер статьи47
Число страниц23
ЖурналPlants
Том8
Номер выпуска2
DOI
СостояниеОпубликовано - 1 фев 2019

Отпечаток

phloem
flax
microRNA
stem
stems
genes
fiber crops
leucine zipper
Linum usitatissimum
plant architecture
gene
parenchyma (plant tissue)
lasers
xylem
proteins
developmental stages
analysis
fibre
protein
developmental stage

Предметные области Scopus

  • Экология, эволюция поведение и систематика
  • Прикладная ботаника
  • Экология

Цитировать

Gorshkov, O., Chernova, T., Mokshina, N., Gogoleva, N., Suslov, D., Tkachenko, A., & Gorshkova, T. (2019). Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. Plants, 8(2), [47]. https://doi.org/10.3390/plants8020047
Gorshkov, Oleg ; Chernova, Tatyana ; Mokshina, Natalia ; Gogoleva, Natalia ; Suslov, Dmitry ; Tkachenko, Alexander ; Gorshkova, Tatyana. / Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. В: Plants. 2019 ; Том 8, № 2.
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title = "Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles",
abstract = "Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, andmiR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.",
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Gorshkov, O, Chernova, T, Mokshina, N, Gogoleva, N, Suslov, D, Tkachenko, A & Gorshkova, T 2019, 'Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles', Plants, том. 8, № 2, 47. https://doi.org/10.3390/plants8020047

Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. / Gorshkov, Oleg; Chernova, Tatyana; Mokshina, Natalia; Gogoleva, Natalia; Suslov, Dmitry; Tkachenko, Alexander; Gorshkova, Tatyana.

В: Plants, Том 8, № 2, 47, 01.02.2019.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles

AU - Gorshkov, Oleg

AU - Chernova, Tatyana

AU - Mokshina, Natalia

AU - Gogoleva, Natalia

AU - Suslov, Dmitry

AU - Tkachenko, Alexander

AU - Gorshkova, Tatyana

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, andmiR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.

AB - Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, andmiR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.

KW - Flax

KW - Intrusive growth

KW - Laser microdissection

KW - MiRNA

KW - Phloem fibers

KW - Transcriptome

KW - LINUM-USITATISSIMUM

KW - ARABIDOPSIS-THALIANA

KW - READ ALIGNMENT

KW - IN-SILICO IDENTIFICATION

KW - RESPONSE REGULATORS

KW - transcriptome

KW - CELL-PROLIFERATION

KW - MICRORNAS

KW - flax

KW - intrusive growth

KW - GENE-EXPRESSION

KW - phloem fibers

KW - TARGETS

KW - laser microdissection

KW - miRNA

KW - DIFFERENTIATION

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U2 - 10.3390/plants8020047

DO - 10.3390/plants8020047

M3 - Article

C2 - 30791461

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Gorshkov O, Chernova T, Mokshina N, Gogoleva N, Suslov D, Tkachenko A и соавт. Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. Plants. 2019 Февр. 1;8(2). 47. https://doi.org/10.3390/plants8020047