TY - JOUR

T1 - Indoleacetic acid levels in wheat and rice seedlings under oxygen deficiency and subsequent reoxygenation

AU - Yemelyanov, Vladislav V.

AU - Lastochkin, Victor V.

AU - Chirkova, Tamara V.

AU - Lindberg, Sylvia M.

AU - Shishova, Maria F.

N1 - Yemelyanov, V.V.; Lastochkin, V.V.; Chirkova, T.V.; Lindberg, S.M.; Shishova, M.F. Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation. Biomolecules 2020, 10, 276.

PY - 2020/2

Y1 - 2020/2

N2 - The lack of oxygen and post-anoxic reactions cause significant alterations of plant growth and metabolism. Plant hormones are active participants in these alterations. This study focuses on auxin–a phytohormone with a wide spectrum of effects on plant growth and stress tolerance. The indoleacetic acid (IAA) content in plants was measured by ELISA. The obtained data revealed anoxia-induced accumulation of IAA in wheat and rice seedlings related to their. tolerance of oxygen deprivation. The highest IAA accumulation was detected in rice roots. Subsequent reoxygenation was accompanied with a fast auxin reduction to the control level. A major difference was reported for shoots: wheat seedlings contained less than one-third of normoxic level of auxin during post-anoxia, while IAA level in rice seedlings rapidly recovered to normoxic level. It is likely that the mechanisms of auxin dynamics resulted from oxygen-induced shift in auxin degradation and transport. Exogenous IAA treatment enhanced plant survival under anoxia by decreased electrolyte leakage, production of hydrogen peroxide and lipid peroxidation. The positive effect of external IAA application coincided with improvement of tolerance to oxygen deprivation in the 35S:iaaM × 35S:iaaH lines of transgene tobacco due to its IAA overproduction.

AB - The lack of oxygen and post-anoxic reactions cause significant alterations of plant growth and metabolism. Plant hormones are active participants in these alterations. This study focuses on auxin–a phytohormone with a wide spectrum of effects on plant growth and stress tolerance. The indoleacetic acid (IAA) content in plants was measured by ELISA. The obtained data revealed anoxia-induced accumulation of IAA in wheat and rice seedlings related to their. tolerance of oxygen deprivation. The highest IAA accumulation was detected in rice roots. Subsequent reoxygenation was accompanied with a fast auxin reduction to the control level. A major difference was reported for shoots: wheat seedlings contained less than one-third of normoxic level of auxin during post-anoxia, while IAA level in rice seedlings rapidly recovered to normoxic level. It is likely that the mechanisms of auxin dynamics resulted from oxygen-induced shift in auxin degradation and transport. Exogenous IAA treatment enhanced plant survival under anoxia by decreased electrolyte leakage, production of hydrogen peroxide and lipid peroxidation. The positive effect of external IAA application coincided with improvement of tolerance to oxygen deprivation in the 35S:iaaM × 35S:iaaH lines of transgene tobacco due to its IAA overproduction.

KW - Auxin

KW - Oxygen deficiency

KW - Post-anoxia

KW - Rice

KW - Tobacco

KW - Wheat

KW - ABIOTIC STRESS

KW - auxin

KW - ADVENTITIOUS ROOT-FORMATION

KW - PETIOLE ELONGATION

KW - rice

KW - CELL-DEATH

KW - PLANT ADAPTATION

KW - ETHYLENE

KW - SUBMERGENCE

KW - post-anoxia

KW - RUMEX

KW - GROWTH

KW - tobacco

KW - wheat

KW - oxygen deficiency

KW - AUXIN-TRANSPORT

UR - http://www.scopus.com/inward/record.url?scp=85079334576&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/dcaa2f3a-c776-37dc-b213-b4a0e542d0a2/

U2 - 10.3390/biom10020276

DO - 10.3390/biom10020276

M3 - Article

C2 - 32054127

AN - SCOPUS:85079334576

VL - 10

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 2

M1 - 276

ER -