Homogeneous liquid-liquid microextraction based on primary amine phase separation: a novel approach for sample pretreatment

Результат исследований: Научные публикации в периодических изданияхстатьярецензирование

3 Цитирования (Scopus)

Аннотация

A homogeneous liquid-liquid microextraction procedure based on primary amine phase separation was developed as a novel approach for the pretreatment of biological fluids. The procedure assumes primary amine dissolution in the aqueous sample phase, resulting in the creation of a homogeneous sample solution followed by phase separation and analyte extraction in the presence of a polar, water-miscible organic solvent. The phenomenon of primary amine phase separation from homogeneous aqueous solution in the presence of a polar, water-miscible organic solvent was studied in detail and applied for separation and preconcentration in chemical analysis for the first time. The suggested approach was used for the HPLC-UV determination of meropenem as a proof-of-concept analyte in human plasma and serum samples. Under optimal conditions, the detector response of meropenem was linear in the concentration range of 0.1–100.0 mg L −1. The limit of detection was calculated from a blank test based on 3σ, was 0.03 mg L −1.

Язык оригиналаанглийский
Страницы (с-по)117-122
Число страниц6
ЖурналAnalytica Chimica Acta
Том1074
DOI
СостояниеОпубликовано - 2019

Предметные области Scopus

  • Аналитическая химия
  • Биохимия
  • Химия окружающей среды
  • Спектроскопия

Fingerprint Подробные сведения о темах исследования «Homogeneous liquid-liquid microextraction based on primary amine phase separation: a novel approach for sample pretreatment». Вместе они формируют уникальный семантический отпечаток (fingerprint).

Цитировать