Histone tails in nucleosome: fuzzy interaction with DNA

S. Rabdano, M. Shannon, S. Izmailov, N. Gonzalez Salguero, M. Zandian, M. Poirier, N. Skrynnikov, Christopher Jaroniec

Результат исследований: Научные публикации в периодических изданияхтезисы

Выдержка

New evidence from NMR spectroscopy suggests that histone tails remain
highly dynamic even in the condensed state of chromatin. To probe the
dynamic behavior of H4 histone N-terminal tail in greater detail, we
prepared a sample of mononucleosome containing 15N, 13 C-labeled H4
histone. The HSQC spectrum of this sample features observable signals
from the first fifteen residues in H4; half of these signals have been
successfully assigned and used for site-specific 15N relaxation
measurements. The experimentally obtained chemical shifts and relaxation
rates paint the picture of moderately mobile H4 tail with random-coil-like
conformational properties. We have also recorded a μs-long MD trajectory
of mononucleosome in the explicit TIP4P-D solvent, which has been
designed specifically for (partially) disordered protein systems. This
trajectory successfully reproduced the experimentally measured chemical
shifts and relaxation rate constants. According to the MD data, the positively
charged H4 tail hovers over the negatively charged ds-DNA, making
transient contacts with both DNA backbone and major/minor grooves. This
type of behavior, underpinned by electrostatic attraction and characterized
by substantial mobility of H4 tail relative to the DNA chain, can be
classified as "fuzzy interaction".
Язык оригиналаанглийский
Страницы (с-по)48
ЖурналEuropean Biophysics Journal
Том48
Номер выпускаSuppl 1
СостояниеОпубликовано - 1 июл 2019

Цитировать

Rabdano, S., Shannon, M., Izmailov, S., Gonzalez Salguero, N., Zandian, M., Poirier, M., ... Jaroniec, C. (2019). Histone tails in nucleosome: fuzzy interaction with DNA. European Biophysics Journal, 48(Suppl 1), 48.
Rabdano, S. ; Shannon, M. ; Izmailov, S. ; Gonzalez Salguero, N. ; Zandian, M. ; Poirier, M. ; Skrynnikov, N. ; Jaroniec, Christopher. / Histone tails in nucleosome: fuzzy interaction with DNA. В: European Biophysics Journal. 2019 ; Том 48, № Suppl 1. стр. 48.
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title = "Histone tails in nucleosome: fuzzy interaction with DNA",
abstract = "New evidence from NMR spectroscopy suggests that histone tails remainhighly dynamic even in the condensed state of chromatin. To probe thedynamic behavior of H4 histone N-terminal tail in greater detail, weprepared a sample of mononucleosome containing 15N, 13 C-labeled H4histone. The HSQC spectrum of this sample features observable signalsfrom the first fifteen residues in H4; half of these signals have beensuccessfully assigned and used for site-specific 15N relaxationmeasurements. The experimentally obtained chemical shifts and relaxationrates paint the picture of moderately mobile H4 tail with random-coil-likeconformational properties. We have also recorded a μs-long MD trajectoryof mononucleosome in the explicit TIP4P-D solvent, which has beendesigned specifically for (partially) disordered protein systems. Thistrajectory successfully reproduced the experimentally measured chemicalshifts and relaxation rate constants. According to the MD data, the positivelycharged H4 tail hovers over the negatively charged ds-DNA, makingtransient contacts with both DNA backbone and major/minor grooves. Thistype of behavior, underpinned by electrostatic attraction and characterizedby substantial mobility of H4 tail relative to the DNA chain, can beclassified as {"}fuzzy interaction{"}.",
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Rabdano, S, Shannon, M, Izmailov, S, Gonzalez Salguero, N, Zandian, M, Poirier, M, Skrynnikov, N & Jaroniec, C 2019, 'Histone tails in nucleosome: fuzzy interaction with DNA', European Biophysics Journal, том. 48, № Suppl 1, стр. 48.

Histone tails in nucleosome: fuzzy interaction with DNA. / Rabdano, S.; Shannon, M.; Izmailov, S.; Gonzalez Salguero, N.; Zandian, M.; Poirier, M.; Skrynnikov, N.; Jaroniec, Christopher.

В: European Biophysics Journal, Том 48, № Suppl 1, 01.07.2019, стр. 48.

Результат исследований: Научные публикации в периодических изданияхтезисы

TY - JOUR

T1 - Histone tails in nucleosome: fuzzy interaction with DNA

AU - Rabdano, S.

AU - Shannon, M.

AU - Izmailov, S.

AU - Gonzalez Salguero, N.

AU - Zandian, M.

AU - Poirier, M.

AU - Skrynnikov, N.

AU - Jaroniec, Christopher

PY - 2019/7/1

Y1 - 2019/7/1

N2 - New evidence from NMR spectroscopy suggests that histone tails remainhighly dynamic even in the condensed state of chromatin. To probe thedynamic behavior of H4 histone N-terminal tail in greater detail, weprepared a sample of mononucleosome containing 15N, 13 C-labeled H4histone. The HSQC spectrum of this sample features observable signalsfrom the first fifteen residues in H4; half of these signals have beensuccessfully assigned and used for site-specific 15N relaxationmeasurements. The experimentally obtained chemical shifts and relaxationrates paint the picture of moderately mobile H4 tail with random-coil-likeconformational properties. We have also recorded a μs-long MD trajectoryof mononucleosome in the explicit TIP4P-D solvent, which has beendesigned specifically for (partially) disordered protein systems. Thistrajectory successfully reproduced the experimentally measured chemicalshifts and relaxation rate constants. According to the MD data, the positivelycharged H4 tail hovers over the negatively charged ds-DNA, makingtransient contacts with both DNA backbone and major/minor grooves. Thistype of behavior, underpinned by electrostatic attraction and characterizedby substantial mobility of H4 tail relative to the DNA chain, can beclassified as "fuzzy interaction".

AB - New evidence from NMR spectroscopy suggests that histone tails remainhighly dynamic even in the condensed state of chromatin. To probe thedynamic behavior of H4 histone N-terminal tail in greater detail, weprepared a sample of mononucleosome containing 15N, 13 C-labeled H4histone. The HSQC spectrum of this sample features observable signalsfrom the first fifteen residues in H4; half of these signals have beensuccessfully assigned and used for site-specific 15N relaxationmeasurements. The experimentally obtained chemical shifts and relaxationrates paint the picture of moderately mobile H4 tail with random-coil-likeconformational properties. We have also recorded a μs-long MD trajectoryof mononucleosome in the explicit TIP4P-D solvent, which has beendesigned specifically for (partially) disordered protein systems. Thistrajectory successfully reproduced the experimentally measured chemicalshifts and relaxation rate constants. According to the MD data, the positivelycharged H4 tail hovers over the negatively charged ds-DNA, makingtransient contacts with both DNA backbone and major/minor grooves. Thistype of behavior, underpinned by electrostatic attraction and characterizedby substantial mobility of H4 tail relative to the DNA chain, can beclassified as "fuzzy interaction".

M3 - Meeting Abstract

VL - 48

SP - 48

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - Suppl 1

ER -

Rabdano S, Shannon M, Izmailov S, Gonzalez Salguero N, Zandian M, Poirier M и соавт. Histone tails in nucleosome: fuzzy interaction with DNA. European Biophysics Journal. 2019 Июль 1;48(Suppl 1):48.