Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism

Ivan M. Pchelin, Yuri V. Mochalov, Daniil V. Azarov, Sofya A. Romanyuk, Galina A. Chilina, Irina V. Vybornova, Tatiyana V. Bogdanova, Vasily V. Zlatogursky, Svetlana V. Apalko, Natalia V. Vasilyeva, Anastasia E. Taraskina

Результат исследований: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population. Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure. Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction. Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5’-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity. Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.

Язык оригиналаанглийский
Страницы (с-по)1244-1254
Число страниц11
ЖурналMycoses
Том63
Номер выпуска11
DOI
СостояниеОпубликовано - 1 ноя 2020

Предметные области Scopus

  • Дерматология
  • Инфекционные заболевания

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