Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique

Результат исследований: Научные публикации в периодических изданияхстатья

2 Цитирования (Scopus)

Выдержка

The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability
Язык оригиналаанглийский
Страницы (с-по)4587–4593
ЖурналParasitology Research
Том115
Номер выпуска12
DOI
СостояниеОпубликовано - 2016

Отпечаток

Echinostomatidae
Cercaria
Trematoda
polymorphism
Clone Cells
genetic polymorphism
clones
cercariae
genomics
Parthenogenesis
Reproduction
Littorina littorea
methodology
Alleles
parthenogenesis
Himasthla elongata
alleles

Цитировать

@article{65eeb2cadde54c918976944d27c92798,
title = "Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique",
abstract = "The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability",
keywords = "AFLP, H. elongata, parthenitae, Clonal diversity, Monomiracidial infection",
author = "N.K. Galaktionov and O.I. Podgornaya and P.P. Strelkov and Galaktionov, {K. V.}",
year = "2016",
doi = "10.1007/s00436-016-5249-1",
language = "English",
volume = "115",
pages = "4587–4593",
journal = "Parasitology Research",
issn = "0932-0113",
publisher = "Springer",
number = "12",

}

Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique. / Galaktionov, N.K.; Podgornaya, O.I.; Strelkov, P.P.; Galaktionov, K. V.

В: Parasitology Research, Том 115, № 12, 2016, стр. 4587–4593.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique

AU - Galaktionov, N.K.

AU - Podgornaya, O.I.

AU - Strelkov, P.P.

AU - Galaktionov, K. V.

PY - 2016

Y1 - 2016

N2 - The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability

AB - The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability

KW - AFLP

KW - H. elongata

KW - parthenitae

KW - Clonal diversity

KW - Monomiracidial infection

U2 - 10.1007/s00436-016-5249-1

DO - 10.1007/s00436-016-5249-1

M3 - Article

VL - 115

SP - 4587

EP - 4593

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

IS - 12

ER -