© 2014, Pleiades Publishing, Ltd. The horizontal transfer of genes into the nuclear genome of plants is a rare phenomenon. Currently, the only known examples of this have been described for several Nicotiana and Linaria species. In the genome of Linaria dalmatica (L.) Mill a nucleotide sequence sharing 94% similarity with the sequence of the Agrobacterium rhizogenes rolC gene have been described for the first time. The significance of the rolC gene and T-DNA for the evolution of Linaria is discussed, as well as their role in the origin of plant species.