Genetic analysis of spontaneous suppressors of the Pho85 mutation in yeast Saccharomyces cerevisiae

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Chaperones are known to play an important role in complexation of cyclin-dependent kinases with cyclins. In yeast cells growing in the presence of phosphate, cyclin-dependent kinase Pho85p and cyclin Pho80p form a complex and phosphorylate activator Pho4p. As a result, Pho4p is exported from the nucleus, and the PHO5 gene is not transcribed. The mutations suppressing the pho85 mutation were analyzed in order to identify genes which code for chaperones involved in the formation of the Pho80p-Pho85p complex in the presence of environmental phosphate. Dominant mutations DSP1, DSP2, and DSP4-6 were found. It is shown that the DSP1 gene is 2.1 cM away from the PHO85 gene on chromosome XVI and probably coincides with the EGD1 gene coding for a chaperone.

Язык оригиналаанглийский
Страницы (с-по)12-17
Число страниц6
ЖурналRussian Journal of Genetics
Том39
Номер выпуска1
DOI
СостояниеОпубликовано - 1 янв 2003

Отпечаток

Genetic Suppression
Saccharomyces cerevisiae
Yeasts
Cyclins
Cyclin-Dependent Kinases
Genes
Mutation
Phosphates
Chromosomes

Предметные области Scopus

  • Генетика

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abstract = "Chaperones are known to play an important role in complexation of cyclin-dependent kinases with cyclins. In yeast cells growing in the presence of phosphate, cyclin-dependent kinase Pho85p and cyclin Pho80p form a complex and phosphorylate activator Pho4p. As a result, Pho4p is exported from the nucleus, and the PHO5 gene is not transcribed. The mutations suppressing the pho85 mutation were analyzed in order to identify genes which code for chaperones involved in the formation of the Pho80p-Pho85p complex in the presence of environmental phosphate. Dominant mutations DSP1, DSP2, and DSP4-6 were found. It is shown that the DSP1 gene is 2.1 cM away from the PHO85 gene on chromosome XVI and probably coincides with the EGD1 gene coding for a chaperone.",
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Genetic analysis of spontaneous suppressors of the Pho85 mutation in yeast Saccharomyces cerevisiae. / Sambuk, E. V.; Popova, Yu G.; Padkina, M. V.

В: Russian Journal of Genetics, Том 39, № 1, 01.01.2003, стр. 12-17.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Genetic analysis of spontaneous suppressors of the Pho85 mutation in yeast Saccharomyces cerevisiae

AU - Sambuk, E. V.

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AU - Padkina, M. V.

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AB - Chaperones are known to play an important role in complexation of cyclin-dependent kinases with cyclins. In yeast cells growing in the presence of phosphate, cyclin-dependent kinase Pho85p and cyclin Pho80p form a complex and phosphorylate activator Pho4p. As a result, Pho4p is exported from the nucleus, and the PHO5 gene is not transcribed. The mutations suppressing the pho85 mutation were analyzed in order to identify genes which code for chaperones involved in the formation of the Pho80p-Pho85p complex in the presence of environmental phosphate. Dominant mutations DSP1, DSP2, and DSP4-6 were found. It is shown that the DSP1 gene is 2.1 cM away from the PHO85 gene on chromosome XVI and probably coincides with the EGD1 gene coding for a chaperone.

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