Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val.

Polina Klauzen, Kseniya Perepelina, Aleksandr Khudiakov, Anna Zlotina, Yulia Fomicheva, Tatiana Pervunina, Tatiana Vershinina, Anna Kostareva, Anna Malashicheva

Результат исследований: Научные публикации в периодических изданияхстатья

Аннотация

LMNA mutations are often linked to laminopathies characterized by tissue-specific disorders. We generated two induced pluripotent stem cells lines from patient carrying genetic variant LMNA p.Asp357Val associated with paroxysmal ventricular tachycardia and myopathy. Reprogramming of patient's peripheral blood mononuclear cells was performed using Sendai viruses. Characterization of the FAMRCi005-A and FAMRCi005-B lines revealed that generated iPSC lines expressed pluripotent stem cell markers, had normal karyotype and demonstrated triliniage differentiation ability. Generated cell lines can be used to investigate the molecular links between LMNA genetic variants and cardiac disorders.

Язык оригиналаанглийский
Номер статьи101719
ЖурналStem Cell Research
Том43
DOI
СостояниеОпубликовано - мар 2020

Предметные области Scopus

  • Биология развития
  • Клеточная биология

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