Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene

Aleksandr Khudiakov, Daria Kostina, Anna Zlotina, Tatiana Nikulina, Alexey Sergushichev, Alexandra Gudkova, Alexey Tomilin, Anna Malashicheva, Anna Kostareva

Результат исследований: Научные публикации в периодических изданияхстатья

3 Цитирования (Scopus)

Аннотация

Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.

Язык оригиналаанглийский
Страницы (с-по)77-80
Число страниц4
ЖурналStem Cell Research
Том24
DOI
СостояниеОпубликовано - 1 окт 2017

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Предметные области Scopus

  • Биология развития
  • Клеточная биология

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